ORIGINAL ARTICLE
Effects of Artemisia annua methanolic extract on the enzymatic components of intermediary metabolism and the antioxidant system of Pseudococcus viburni Signoret
1
Tea Research Center, Horticulture Science Research Institute, Agricultural Research,
Education and Extension Organization (AREEO), Lahijan, Iran
2
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3
Plant Protection Department, Research Center for Agriculture and Natural Resources, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-01-09
Acceptance date: 2018-04-23
Online publication date: 2018-10-18
Corresponding author
Samar Ramzi
Tea Research Center, Horticulture Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Sheikh Zahed Street, 41362536521 Lahijan, Iran
Tea Research Center, Horticulture Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Sheikh Zahed Street, 41362536521 Lahijan, Iran
Journal of Plant Protection Research 2018;58(3):289-296
KEYWORDS
TOPICS
ABSTRACT
Toxicity and physiological alterations were determined in Pseudococcus viburni nymphs treated with Artemisia annua methanolic extract. The leaf dipping bioassay showed LC50
values of 0.287% and 0.194% 24 and 48 hours post-exposure. Activities of general esterases were significantly higher in the control nymphs than in those which had been treated except for the 48 h time interval using α-naphtyl acetate. The activity of glutathione
S-transferase using CDNB (1-chloro-2,4-dinitrobenzene) in the control nymphs, was significantly higher than in the control at both time intervals while no significant difference was observed after 24 h in addition to the higher enzymatic activity in the treated nymphs after 48 h. All three aminotransferases were significantly more active in the control nymphs except for time intervals of 24 h for γ-glutamyl transferase and 48 h for alanine aminotransferase.
Higher activities of lactate dehydrogenase, acid- and alkaline phosphatase were found in the control nymphs than in treated nymphs for all time intervals. Activities of the enzymes involved in the antioxidant system including catalase, peroxidase, superoxide
dismutase, ascorbate peroxidase and glucose-6-phosphate dehydrogenase was increased in the treated nymphs compared to the control. Results of the current study demonstrated toxic effects of A. annua methanolic extract on P. viburni nymphs causing mortality and physiological turbulences.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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