ORIGINAL ARTICLE
Effect of formulating of Beauveria bassiana conidia on their viability and pathogenicity to the onion thrips, Thrips tabaci Lind. (Thysanoptera: Thripidae)
1
Department of Plant Protection, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Corresponding author
Reyhaneh Ezzati-Tabrizi
Department of Plant Protection, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Department of Plant Protection, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Journal of Plant Protection Research 2009;49(1):97-104
KEYWORDS
TOPICS
ABSTRACT
The wettable powder was prepared on the basis of aerial conidia of
two isolates
of the entomopathognic fungus
,
Beauveria
bassiana
. Viability and pathogenicity of conidia products were evaluated
against the second-instar larva of
Thrips tabaci in four cases; Conidial-product Maintained in Refrigerator (CMR), Conidial-product Maintained in Laboratory (CML), New Formulated Conidia
(NFC) and New Conidia without formulation (NC). Analysis of corrected seven-day total mortality data revealed that there were significant differences among these product-cases in their pathogenicity to thrips larvae. Recorded mortality rates for CMR, CML, NFC and NC showed that the pathogenicity of CML was lower compared to three other cases for both isolates. In the next step, inorganic salts (MgCl2, NH4PO4, KH2PO4, MgSO4 and NaCl) were added at a rate of 0.1 M into the both CMR and CML products. Bioassay results indicated that caused total mortality of thrips larvae increased with adding of salts. Our results showed that applied carriers and salts have positively effected preserving of conidia viability and pathogenicity to the second-instar larva of the onion thrips.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (49)
1.
Abadias M., Benabarre A., Teixido N., Usall J., Vinas I. 2001. Effect of freeze drying and protectants on viability of the biocontrol yeast Candida sake. Inter. J. Food. Microbiol. 65: 173–182.
2.
Alves S.B., Silveira-Neto S., Pereira R.M., Macedop N. 1987. Study of formulation of Metarhizium anisopliae (Metsch.) Sorok. in different storage conditions. Ecossistema 12: 78–87.
3.
Bateman R.P. 1992. Controlled droplet application of mycopesticides to locusts and grasshoppers. CAB International, Wallingford, UK: 249–254.
4.
Batta Y.A. 2004. Control of the rice weevil Sitophilus oryzae(Coleoptera: Curculionidae) with various formulations of Metarhizium anisopliae. Crop Prot. 23: 103–108.
5.
Behle R., Compton D., Laszlo J., Shapiro Ilan D. 2006. Potential use of soyscreen in formulations of Beauveria bassiana for UV protection. Entomol. Soci. Amer. Ann. Meet. North Central Branch. Paper No. D-215.
6.
Beker M.J., Rapoport A.I. 1987. Conservation of yeast by dehydration. Adv. Bioch. Eng. Biotechnol. 35: 127–171.
7.
Bhattacharya S.K., Basu M.K. 1982. Kaolin powder as a fungal carrier. Appl. Environ. Microbiol. 44: 751–753.
8.
Burges H.D. 1998. Formulation of mycoinsecticides. p. 131–185.In: “ Formulation of Microbial Biopesticides” (H.D. Burges, ed.). Kluwer Academic Publishes, Dordercht. Champagne C.P., Gardner N., Brochu E., Beaulieu Y. 1991. The freeze drying of lactic acid bacteria: a review. Can. Inst. Sci. Technol. 24: 118–128.
9.
Chen A., Shi Z., Zhang L. 2008. The effects of some storage conditions on viability of Lecanicillium lecanii conidia to white fly (Homoptera: Trialeurodes vaporariorum). Biocontrol Sci. Technol. 18: 267–278.
10.
Connick Jr W.J., Jackson M.A., Williams K.S., Boyette C.D. 1997. Stability of microsclerotial inoculum of Colletotrichum truncatum encapsulated in wheat flour-kaolin granules. World. Microbiol. Biotechnol. 13: 549–554.
11.
Costa E., Teixidó N., Usall J., Fons E., Gimeno V., Delgado J., Viñas I. 2001. Survival of Pantoea agglomerans strain CPA-2 in spray-drying process. J. Food Prot. 65: 185–191.
12.
Daoust R.A., Roberts D.W. 1983. Studies on prolonged storage of Metarhizium anisopliae conidia: effect of temperature and relative humidity on conidial viability and virulence against mosquitoes. J. Invertebr. Pathol. 41: 143–150.
13.
De Cal A., Larena I., Guijarro B., Melgarejo P. 2002. Solid state fermentation to produce conidia of Penicillium frequentans, a biocontrol agent against brown rot on stone fruits. Biocontrol Sci. Technol.12: 715–725.
14.
Dresner E. 1949. Culture and use of entomogenous fungi for the control of insects. Contr. Boyce Thompson Inst. 15: 319–335.
15.
Edgington S., Segura H., Dela Rosa W., Williams T. 2000. Photoprotection of Beauveria bassiana: testing simple formulations for control of the berry borer. J. Pest. Manag. 46: 169–176.
16.
Elzein A., Kroschel J., Müller-Stover D. 2004. Effects of inoculum type and propagule concentration on shelf-life of Pesta formulations containing Fusarium oxysporum Foxy 2, a potential mycoherbicide agent for Strigaspp. Biol. Control. 30: 203–211.
17.
Feng M.G., Poprawski T.J., Khachatourians G.G. 1994. Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status. Biocontrol Sci. Technol. 4: 3–34.
18.
Guijarro B., Melgarejo P., De Cal A. 2007. Effect of stabilizers on the shelf-life of Penicillium frequentans conidia and their efficacy as a biological agent against peach brown rot. J. Food. Microbiol. 113: 117–124.
19.
Hardy VG., Teakle DS. 1992. Transmission of sowbane mosaic virus by Thrips tabaci in the presence and absence of virus carrying pollen. Ann. Appl. Biol. 121: 315–320.
20.
Herron G.A., James T.M. 2005. Monitoring insecticide in Australian Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) detects fipronil and spinosad resistance. Aust. J. Entomol. 44: 299–303.
21.
Hong T.D., Edgingron S., Ellis R.H., Aquino de Muro M., Moore D. 2005. Saturated salt solutions for humidity control and the survival of dry powder and oil formulations of Beauveria bassiana conidia. J. Invertebr. Pathol. 89: 136–143.
22.
Jackson M.A., Erhan S. 2006. Influence of formulation additives on the desiccation tolerance and storage stability of blastospores of the entomopathogenic fungus Paecilpmyces fumosoroseus. Biocontrol Sci. Technol. 16: 61–75.
23.
Jiang Q., Ying SH-H., Feng M-G. 2007. Enhanced frequency of Beauveria bassiana blastospore transformation by restriction enzyme-mediated integration and electroporation. J. Microbiol. Methods. 69: 512–517.
24.
Jones K.A., Burges H.D. 1998. Technology of formulation and application. p. 7–30.In: “Formulation of Microbial Biopesticides” (H.D. Burges, ed.). Kluwer Academic Publishers, Dordrecht, The Netherlands.
25.
Kucuk C., Kivanc M. 2005. Effect of formulation on the viability of biocontrol agent, Trichoderma harzianum conidia. Afric. J. Biotechnol.4: 483–486.
26.
Larena I., De Cal A., Melgarejo P. 2007. Effect of stabilizers on shelf-life of Epicoccum nigrum formulations and their relationship with biocontrol of post harvest brown rot by Monilinia of peaches. J. Appl. Microbiol. 102: 570–582.
27.
Larena I., Melgarejo P., De Cal A. 2002. Production, survival and evaluation of solidsubstrate inocula of Penicillium oxalicum, a biocontrol agent against Fusarium wilt of tomato. Phytopathology 92: 863–869.
28.
Leora Software. 1987. POLO-PC a User’s Guide to Probit or Logit Analysis. Leora Software, Berkeley, CA.
30.
Lievense L.C., Van’t Riet K. 1994. Convective drying of bacteria. II. Factors influencing survival. Adv. Bioch.Eng. Biotechnol. 51: 72–89.
31.
Luz Ch., Silva I.G., Magalhaes B.P., Cordeiro C.M.T., Tigano M.S. 1999. Control of Triatoma infestans (Klug) (Reduviidae: Triatominae) with Beauveria bassiana(Bals.) Vuill.: Preliminary assays on formulation and application in the field. An. Soc. Entomol. Brasil 28: 101–110.
32.
Marques J.E., Alves B.S., Marques R.M.I. 1999. Effects of the temperature and storage on formulation with mycelia of Beauveria bassiana(Bals.) Vuill and Metarrhizium anisopliae(Metschn.) Sorok. Bars. Arch. Biol. Technol. 42: 153–160.
33.
Moore D., Bateman R.P., Crey M., Prior C. 1995. Long term of storage of Metarhizium flavoviridae conidia in oil formulation for the control of locusts and grasshoppers. Biocontrol Sci. Technol. 5: 193–199.
34.
Nagayama K., Watanabe S., Kumakura K., Ichikawa T., Makino T. 2007. Development and commercialization of Trichoder ma asperellumSKT-1 (Ecohope®), a microbial pesticide. J. Pest. Sci. 32: 141–142.
35.
Polar P., Kairo M.T.K., Moore D., Pegram R., John S-A. 2005. Comparison of water, oils and emulsifiable adjuvant oils as formulating agents for Metarhizium anisopliae for use in control of Boophilus microplus. Mycopathologia 160: 151–157.
36.
Pourian H.R., Ezzati-Tabrizi R., Talaei-Hassanloui R. 2008. An improved cage system for the bioassay of Metarhizium anisopliae on Thrips tabaci(Thysanoptera: Thripidae). Biocontrol Sci. Technol. 18: 745–752.
37.
Püntener W. 1981. Manual for Field Trials in Plant Protection 2nd ed. Agricultural Division, Ciba-Geigy Limited.
38.
Rhodes D.J. 1993. Formulation of biological control agents. p. 411–439. In: “Exploitation of Microorganism” (D.G. Jones, ed.). Chapman and Hall, London.
39.
Sandoval-Coronado C.F., Luna-olvera H.A., Arevalo-Nino K., Jackson M.A., Poprawski T.J. 2001. Drying and formulation of blastospores of Paecilomyces fumosoroseus produced in two different liquid media. World. J. Microbiol. Biotechnol.17: 423–428.
40.
Shabana Y. M., Muller-Stover D., Sauerborn J. 2003. Granular Pesta formulation of Fusarium oxysporum f. sp. orthoceras for biological control of sunflower broomrape: efficacy and shelf-life. Biol. Control. 26: 189–201.
41.
Smith S.M., Moore D., Karanja L.W., Chandi E.A. 1999. Formulation of vegetable fat pellets with pheromone and Beauveria bassiana to control the larger grain borer, Prostephanus truncatus. Pestic. Sci. 55: 711–718.
42.
Stathers T.E., Moore D., Prior C. 1993. The effect of different temperatures on the viability of Metarhizium flavoviridae conidia stored in vegetable and mineral oils. J. Invertebr. Pathol. 62: 111–115.
43.
Steinkraus D.C., Geden G.J., Rutz D.A. 1991. Susceptibility of lesser mealworm to Beauveria bassiana: Effects of host stage, substrate, formulation and host passage. J. Med. Entomol. 28: 314–321.
44.
Stephan D., Zimmermann G. 1998. Development of a spray-drying technique for submerged spores of entomopathogenic fungs. Biocontrol Sci. Technol. 8: 3– 11.
46.
Tadayyon A., Hill G.A., Ingledew W.M., Sokhansanj S. 1997. Contact-Sorption drying of Penicillium bilami in a fluidized bed dryer. J. Chem. Technol. 68: 277–282.
47.
Tanada Y., Kaya H.K. 1993. Insect Pathology. 1st ed. Academic Press, New York, 666 pp.
48.
Teera-Arunsiri A., Suphantharika M., Kentunuti U. 2003. Preparation of spray-Dried wettable Powder formulations of Baillus thuringiensis based biopesticides. J. Eco. Entomol. 96: 292–299.
49.
Ugine T. A., Wright S.P., Sanderson J.P. 2005. Acquisition of lethal doses of Beauveria bassiana conidia by western flower thrips, Frankliniella occidentalis exposed to foliar spray residues of formulated and unformulated conidia. J. Invertebr. Pathol. 90: 10–23.
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