ORIGINAL ARTICLE
Effect of Neotyphodium uncinatum endophyte on meadow fescue yielding, health status and ergovaline production in host-plants
1
University of Technology and Life Sciences in Bydgoszcz
Kordeckiego 20, 85-225 Bydgoszcz, Poland
Department of Phytopathology and Molecular Mycology
2
University of Technology and Life Sciences in Bydgoszcz
Kordeckiego 20, 85-225 Bydgoszcz, Poland
Department of Environmental Chemistry
Corresponding author
Dariusz Pańka
University of Technology and Life Sciences in Bydgoszcz Kordeckiego 20, 85-225 Bydgoszcz, Poland Department of Phytopathology and Molecular Mycology
University of Technology and Life Sciences in Bydgoszcz Kordeckiego 20, 85-225 Bydgoszcz, Poland Department of Phytopathology and Molecular Mycology
Journal of Plant Protection Research 2011;51(4):362-370
KEYWORDS
endophyteNeotyphodium uncinatum (GamsPetrini and Schmidt) GlennBacon and HanlinFestuca pratensisHudspathogensergovalineyield
TOPICS
ABSTRACT
The objective of our research was to assess the beneficial impact of the
Neotyphodium uncinatum
(Gams, Petrini and Schmidt)
Glenn, Bacon and Hanlin endophyte on its natural host – meadow fescue. Assessment was made by measuring the green mass yield,
the susceptibility of the host plants to infection by pathogens, and the content of the toxic alkaloid ergovaline, in field conditions. The
research involved Justa meadow fescue. The studied factors were as follows: endophyte infection (E+ and E-) and system of use (for
pasture and for cut). The effect of
N. uncinatum
on Justa meadow fescue yielding in all the combinations was observed. The presence
of endophyte significantly enhanced higher yields of dry matter compared to the non-infested plants. The infestation of Justa meadow
fescue by the endophyte,
N. uncinatum,
significantly protected the plants from infection of the fungi which causes leaf spot. The endophyte, however, did not affect the development of powdery mildew and rust fungi. Justa meadow fescue showed a relatively high
content of ergovaline when grown in the field. The level of the toxin in the season varies a lot, which suggests a high effect of external
factors on its production. Due to the production of the toxin, the animal feed made from infested plants can pose a threat to animals
when administered over a long period.
N. uncinatum
isolates from Justa meadow fescue cannot be used as biological control agents to
improve the growth and resistance of other cultivars, due to the production of ergovaline
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (37)
1.
An Zhi-Qiang, Siegel M.R., Hollin W., Tsai Huei-Fung, Schmidt D., Schardl C.L. 1993. Relationships among nonAcremonium sp. fungal endophytes in five grass species. Appl. Environ. Microbiol. 59 (5): 1540–1548.
2.
Arechavaleta M., Bacon C.W., Plattner R.D., Hoveland C.S., Radcliffe D.E. 1992. Accumulation of ergopeptide alkaloids in symbiotic tall fescue grown under deficits of soil water and nitrogen fertilizer. Appl. Environ. Microbiol. 58 (3): 857–861.
3.
Bacon C.W., Battista De J. 1991. Endophytic fungi of grasses. p. 231–256. In: “Handbook of Applied Mycology” Vol. 1. “Soil and Plants” (D.K. Arora B. Rai, K.G. Mukerji, G.R. Knudsen, eds.). Marcel Dekker, New York, 720 pp.
4.
Bacon C.W., Richardson M.D., White J.F.Jr. 1997. Modification and uses of endophyte-enhanced turfgrasses: A role for molecular technology. Crop Sci. 37 (5): 1415–1425.
5.
Belesky D.P., Stuedemann J.A., Plattner R.D., Wilkinson S.R. 1988. Ergopeptine alkaloids in grazed tall fescue. Agron. J. 80 (2): 209–212.
6.
Birckenstaedt E., Eickel P., Paul V.H. 1994. Scoring of grass diseases for the evaluation of varieties. IOBC/WPRS Bull. 17 (1): 193–200.
7.
Bouton J., Easton S. 2005. Endophytes in forage cultivars.p. 327–340. In: “Neotyphodium in Cool-Season Grasses” (C.A. Roberts, C.P. West, D.E. Spiers, eds.). Blackwell Publishing, Ames, Iowa, USA, 379 pp.
8.
Bradshaw A.D. 1959. Population differentiation in Agrostis tenis Sibth. II. The incidence and significance of infection by Epichloë typhina. New Phytol. 58 (3): 310–315.
9.
Clarke B.B., White Jr. J.F., Hurley R.H., Torres M.S., Sun S., Huff D.R. 2006. Endophyte mediated suppresion of dollar spot disease in fine fescues. Plant Dis. 90 (8): 994–998.
10.
Clay K. 1984. The effect of the fungus Atkinsonella hypoxylon (Clavicipitaceae) on the reproductive system and demography of the grass Danthonia spicata. New Phytol. 98 (2): 165–175.
11.
Clay K. 1987. Effects of fungal endophytes on the seed and seedling biology of Lolium perenne and Festuca arundinaceae. Oecologia 73 (3): 358–362.
12.
Elmi A.A., West C.P. 1995. Endophyte infection effects on stomatal conductance, osmotic adjustment and drought recovery of tall fescue. New Phytol. 131 (1): 61–67. Faeth S.H. 2002. Are endophytic fungi plant mutualists? Oikos 98 (1): 25–36.
13.
Glenn A.E., Bacon C.W., Price R., Hanlin R.T. 1996. Molecular phylogeny of Acremonium and its taxonomic implications. Mycologia 88 (3): 369–383.
14.
Gwinn K.D., Gavin A.M. 1992. Relationship between endophyte infestation level of tall fescue seed lots and Rhizoctonia zeaeseedling disease. Plant Dis. 76 (9): 911–914.
15.
Johnson M.C., Dahlmann D.L., Siegel M.R., Bush L.P., Latch G.C.M., Potter D.A., Varney D.R. 1985. Insect feeding deterrents in endophyte infected tall fescue. Appl. Environ. Microbiol. 49 (3): 568–571.
16.
Koshino H., Togiya S., Yoshihara T., Sakamura S. 1987. Four fungitoxic C-18 hydroxy unsaturated fatty acids from stromata of Epichloë typhina. Tetrahedron Lett. 28 (1): 73–76.
17.
Latch G.C.M., Christensen M.J. 1982. Ryegrass endophyte, incidence, and control. N. Zeal. J. Agric. Res. 25 (3): 443–448.
18.
Lehtonen P.T., Helander M., Siddiqui S.A., Lehto K., Saikkonen K. 2006. Endophytic fungus decreases plant virus infections in meadow ryegrass (Lolium pratense). Biol. Lett. 2 (4): 620–623.
19.
Lewis G.C. 1996a. A review of research on endophytic fungi worldwide, and its relevance to European grassland, pastures and turf. IOBC/WPRS Bull. 19 (7): 17–24.
20.
Lewis G.C. 1996b. Effect of cutting height on perennial ryegrass with and without infection with endophyte and ryegrass mosaic virus. IOBC/WPRS Bull. 19 (7): 55–58.
21.
Lyons P.C., Evans J.J., Bacon C.W. 1990. Effects of the fungal endophyte Acremonium coenophialum on nitrogen accumulation and metabolism in tall fescue. Plant Physiol. 92 (3): 726–732.
22.
Malinowski D., Leuchtmann A., Schmidt D., Nösberger J. 1997. Growth and water status in meadow fescue is affected by Neotyphodium and Phialophora species endophytes. Agron. J. 89 (4): 673–678.
23.
McCormik M.K., Gross K.L., Smith R.A. 2001. Danthonia spicata(Poaceae) and Atkinsonella hypoxylon(Balansiae): environmental dependance of a symbiosis. Am. J. Bot. 88 (5): 903–909.
24.
Oliver J.W. 2005. Pathophysiologic response to endophyte toxins.p. 291–304. In: “Neotyphodium in Cool-Season Grasses” (C.A. Roberts, C.P. West, D.E. Spiers, eds.). Blackwell Publishing, Ames, Iowa, USA, 379 pp.
25.
Pańka D., Podkówka L., Lamparski R. 2004. Preliminary observations on the resistance of meadow fescue (Festuca pratensis Huds.) infected by Neotyphodium uncinatum to diseases and pests and nutritive value. No. 401. p. 88–90. In: “Proceedings of the 5th International Symposium on Neotyphodium/Grass Interactions” (R. Kallenbach, C. Jr. Rosenkrans, T.R. Lock, eds.). Fayetteville, AR, USA, May 23–26, 2004, 180 pp.
26.
Porter J.K., Thompson F.N. Jr. 1992. Effects of fescue toxicosis on reproduction in livestock. J. Anim. Sci. 70 (5): 1594–1603.
27.
Richardson M.D., Chapman Jr. G.W., Hoveland C.S., Bacon C.W. 1992. Sugar alcohol in endophyte-infected tall fescue under drought. Crop Sci. 32 (4): 1060–1061.
28.
Rottinghaus G.E., Garner G.B., Cornell C.N., Ellis J.L. 1991. HPLC method for quantitating ergovaline in endophyteinfested tall fescue: Seasonal variation of ergovaline levels in stems with leaf sheaths, leaf blades, and seedheads. J. Agric. Food Chem. 39 (1): 112–115.
29.
Saha D.C., Jackson M.A., Johnson-Cicalese J.M. 1988. A rapid staining method for detection of endophytic fungi in turf and forage grasses. Phytopathology 78 (2): 237–239.
30.
Schmidt D. 1994. Influence of endophytes of Festuca pratensis on damping-off diseases of seedlings. IOBC/WPRS Bull. 17 (1): 267–271.
31.
Siegel M.R., Schardll C.L., Philips T.D. 1995. Incidence and compatibility of nonclavicipitaceous fungal endophytes in Festuca and Lolium grass species. Mycologia 87 (2): 196–202.
32.
Smith S.E., Read D.J. 1997. Mycorrhizal Symbiosis. 2nd ed. Academic Press, San Diego, CA, 605 pp.
33.
Thompson F.N., Stuedeman J.A. 1993. Phytophysiology of fescue toxicosis. Agric. Ecosyst. Environ. 44 (1–4): 263–281.
34.
Vincelli P., Powell A.J. 1991. Reaction of perennial ryegrass varieties to red thread, 1990. Biol. Cultural Tests Control Plant Dis. 6, p. 102.
35.
Wäli P.R., Helander M., Nissinen O., Saikkonen K. 2006. Susceptibility of endophyte-infected grasses to winter pathogens (snow molds). Can. J. Bot. 84 (7): 1043–1051.
36.
Wenzel H. 1948. Zur erfassung des schadenausmasses in pflanzenschutzversuchen. Pflanzenschuzberichte 15: 81–84.
37.
Żurek M., Ochodzki P., Wiewióra B. 2010. Ocena zawartości ergowaliny w trawach runi wybranych trwałych użytków zielonych na terenie województwa mazowieckiego. Biul. IHAR 257/258: 39–47.
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