ORIGINAL ARTICLE
Diversity of the fungal community on mango associated with stem end rot and anthracnose diseases based on amplicon targeted metagenomics
1
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
2
Galasari Gunung Sejahtera, Gresik, East Java, Indonesia
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-10-14
Acceptance date: 2023-12-18
Online publication date: 2024-03-06
Corresponding author
Ani Widiastuti
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Journal of Plant Protection Research 2024;64(1):42-51
HIGHLIGHTS
- Fungal community associated to stem end rot is different from anthracnose disease.
- Class Basidiomycota was predominant in the healthy fruits
- Class Ascomycota was predominantly found in the sick fruits
- Lasiodiplodia, Neofusicoccum were found in stem end rot mango
- Colletotrichum was found in the anthracnose symptom and not in the stem end rot
KEYWORDS
Lasiodiplodia theobromaemicrobiome interactionNeofusicoccum cordaticolaNeofusicoccum mangiferaepostharvest disease
TOPICS
ABSTRACT
This study aimed to comprehend the diversity of the fungal community on Chokanan
mango, a premium mango variety from Thailand which is widely cultivated in Indonesia,
associated with stem end rot and anthracnose disease using high-throughput amplicon
targeted metagenomics analysis by next-generation sequencing (NGS). Samples used in
this study were freshly harvested healthy fruits at the age of 15-weeks (H15.ITS), healthy
fruits after 2 weeks incubation (H17.ITS), 17-week old fruits (S17.ITS) with stem end rot
symptoms, and 17-week old fruits (A17.ITS) with anthracnose symptoms. Results showed
that the Basidiomycota phylum was dominant in the healthy fruits, while the Ascomycota
phylum was found dominantly in sick fruits. Based on OTUs alignment of sequenced data,
some species found to be dominantly associated with stem end rot disease in this study
were Lasiodiplodia theobromae, Neofusicoccum cordaticola and N. mangiferae. Dominant
species which were associated with mango anthracnose disease were Colletotrichum gloeosporioides,
Botryosphaeria corticis, Volutella sp., and Pseudofusicoccum violacearum. These
fungal genera were not found to be dominant in healthy fruits at the same age indicating
that specific genera contributed to developing postharvest diseases on mango differently.
The findings confirmed that the fungal community associated with stem end rot and anthracnose
disease on mango was unique, and specific species contributed in particular disease
development. Since mango is an important global commodity, these research findings
will contribute significantly to global biosecurity.
ACKNOWLEDGEMENTS
The authors express their sincere gratitude to Galasari
Gunung Sejahtera, a subsidiary company of Polowijo
Gosari Indonesia Holding which supplied the fruit
samples of Chokanan Mango for this research.
FUNDING
The research
was financially supported by a National Grant
of Basic Research on University 2023 (Penelitian Dasar
Unggulan Perguruan Tinggi/PDUPT 2023) Ministry
of Education, Culture, Research, and Technology,
Indonesia with contract number 018/E5/PG.02.00.
PL/2023 – 2142/UN1/DITLIT/DitLit/PT.01.03/2023.
RESPONSIBLE EDITOR
Sebastian Stenglein
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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