ORIGINAL ARTICLE
Differentiation by simplified AFLP of Pseudomonas syringe pv. syringae isolates from fields, panicles and nurseries of the Guilan Province - Iran
1
University of Guilan, Rasht,41635-1314, Iran
Department of Plant Protection, College of Agriculture
2
University of Guilan, Rasht,41635-1314, Iran
Department of Plant Breeding, College of Agriculture Rasht
3
University of Guilan, Rasht,41635-1314, Iran
Research Institute of Rice, Rasht, 41996-13475, Iran
Corresponding author
Roghayeh Habibi
University of Guilan, Rasht,41635-1314, Iran Department of Plant Protection, College of Agriculture
University of Guilan, Rasht,41635-1314, Iran Department of Plant Protection, College of Agriculture
Journal of Plant Protection Research 2012;52(2):268-274
KEYWORDS
TOPICS
ABSTRACT
Pseudomonas syringae
pv
. syringae
is a plant pathogen bacterium in rice which causes bacterial sheath rot. In this study, simplified AFLP (Amplified fragment length polymorphism) was tested in order to evaluate genetic diversity of 38
Pseudomonas syringae
pv
. syringae
strains isolated from rice fields of the Guilan province, by 12 AFLP primers. The products resulted from AFLP were separated using agarose gel electrophoresis. The maximum number of PIC among all tested primers belonged to primer 36. Based on the
Unweighted Pair Group Method with Arithmetic (UPGMA) method, using NTSISpc Software, all
Pseudomonas syringae
pv
. syringae
strains were divided into three distinct clusters which had a 70% similarity level. Cluster analysis of studied populations (isolates of
fields, panicles, and nurseries) determined that a high genetic identity could be seen among isolates obtained from the panicles and
nurseries, while strains isolated from the fields and nurseries had the most genetic distance with each other. The result of this study
showed that sampling site and weather conditions play an important role in genetic evolution of strains. It was also found that AFLP
is an effective marker in evaluating genetic diversity within and among isolates being studied, while all of them had the same host
and pathogenesis characteristics
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| ISSN: | 1427-4345 |
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