RAPID COMMUNICATION
Development of reverse transcription-loop-mediated isothermal amplification assay for the detection of genetically different isolates of maize dwarf mosaic virus
1
Department of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznan, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-05-25
Acceptance date: 2022-08-08
Online publication date: 2022-09-09
Corresponding author
Katarzyna Trzmiel
Department of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznan, Poland
Department of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznan, Poland
Journal of Plant Protection Research 2022;62(3):302-306
HIGHLIGHTS
- MDMV is a major threat for maize crops worldwide.
- RT-LAMP assay was developed for the first time for MDMV detection.
- Results can be obtained within 1 hr in isothermal conditions.
- Low-cost detection assay can be routinely used in diagnostics of maize crops.
KEYWORDS
TOPICS
ABSTRACT
Maize dwarf mosaic virus (MDMV) is a serious and widespread virus pathogen of maize
plants. This +ssRNA virus belongs to the Potyvirus genus in the Potyviridae family. Together
with sugarcane mosaic virus (SCMV) it causes one of the most important viral diseases
on maize crops in the world – maize dwarf mosaic. Both viruses are transmitted in the
same non-persistent manner by several aphid species. They induce similar symptoms of
leaf mosaic or mottling, stunting and a reduction in plant weight and grain yield. Available
MDMV diagnostics include primarily commercialized enzyme-linked immunosorbent assays
(ELISA) and reverse transcription-polymerase chain reactions (RT-PCR). Here, laborsaving
reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay
was optimized for identification of genetically different MDMV isolates. For this purpose,
primer sets, MDMVF3/MDMVB3 and MDMVFIP/MDMVBIP amplifying fragments of
coat protein coding sequence of MDMV, were used. The specificity of the reaction was
verified using three MDMV (-P1, -Sp, -PV0802-DSMZ) and three SCMV (-P1, -PV0368-
-DSMZ, -PV1207-DSMZ) isolates. Obtained products were visualised by DNA staining,
electrophoretic separation as well as by real-time monitoring of the reaction. The sensitivity
of RT-LAMP and conventional RT-PCR reactions was comparable. Both methods could
detect virus as low as 550 fg · μl–1 of total RNA. This technique has application value for
screening MDMV by phytosanitary services.
ACKNOWLEDGEMENTS
The authors would like to thank Dr Maria Angeles
Achon from the University of Lleida for providing the
MDMV-Sp isolate used in this study.
FUNDING
This study was financed by the Polish Ministry of
Science and Higher Education, by Statutory activity
project: WIB03.
RESPONSIBLE EDITOR
Natasza Borodynko-Filas
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (24)
1.
Achon M., Alonso-Dueñas N., Serrano L. 2011. Maize dwarf mosaic virus diversity in the Johnsongrass native reservoir and in maize: Evidence of geographical, host and temporal differentiation. Plant Pathology 60 (2): 369–377. DOI: https://doi.org/10.1111/j.1365....
2.
Adams M.J., Zebrini F.M., French R., Rabenstein F., Stenger D.C., Valkonen J.P.T. 2012. Potyviridae. p. 1069–1089. In: “Virus Taxonomy, Classification and Nomenclature of Viruses. Ninth report of the International Committee on Taxonomy of Viruses” (A.M.Q. King, M.J. Adams, E.B. Carstens,.
3.
E.J. Lefkowitz, eds). Elsevier Academic Press, Waltham MA. Anandakumar L., Bagyalakshmi K., Raja Muthuramalingam T., Nithya K., Parameswari B., Viswanathan R. 2020. Reverse transcription loop‑mediated isothermal amplification based rapid detection of Sugarcane mosaic virus and Sugarcane streak mosaic virus associated with mosaic disease of sugarcane. Indian Phytopathology 73: 349–358. DOI: https://doi.org/10.1007/s42360....
4.
Budziszewska M., Wieczorek P., Obrępalska-Stęplowska A. 2016. One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus. Archives of Virology 161: 1359–1364. DOI: https://doi.org/10.1007/s00705....
5.
Chen L., Jiao Z., Liu D., Liu X., Xia Z., Deng C., Zhou T., Fan Z. 2017. One-step reverse transcription loop-mediated isothermal amplification for the detection of Maize chlorotic mottle virus in maize. Journal of Virological Methods 240: 49–53. DOI: https://doi.org/10.1016/j.jvir....
6.
Du L., Shi W., Li X., Lan Y., Sung F, Fan Y., Zhou T. 2019. A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting the pathogen of maize rough dwarf disease in China. Australasian Plant Pathology 48: 485–489. DOI: https://doi.org/10.1007/s13313....
7.
Ford R.E., Tosic M., Shukla D.D. 1989. Maize dwarf mosaic virus. AAB Descriptions of Plant Viruses No. 341.
8.
Gordon D.T. 2004. Maize dwarf mosaic. p. 644–649. In: “Viruses and Virus Diseases of Poaceae (Gramineae)” (H. Lapierre, P.A. Signoret, eds.). INRA, Paris, France.
9.
Hall T.A. 1999. BioEdit: a user-friendly biological sequence aligment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
10.
Hasiów-Jaroszewska B., Borodynko N. 2013. Detection of Pepino mosaic virus isolates from tomato by one-step reverse transcription loop-mediated isothermal amplification. Archives of Virology 158: 2153–2156. DOI: https://doi.org/10.1007/s00705....
11.
Hohmann F., Fuchs E., Grüntzig M., Kuntze L. 1996. Development of a routine test for the detection of sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) using tissue print-immunoblotting (TPIB). Journal of Plant Disease and Protection 103 (5): 464–471.
12.
Kannan M., Ismail I., Bunawan H. 2018. Maize dwarf mosaic virus: from genome to disease management. Viruses 10: 492. DOI: https://doi.org/10.3390/v10090....
13.
Keizerweerd A.T., Chandra A., Grisham M.P. 2015. Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Sugarcane mosaic virus and Sorghum mosaic virus in sugarcane. Journal of Virological Methods 212: 23–29. DOI: https://doi.org/10.1016/j.jvir....
14.
Kong P., Steinbiss H.H. 1998. Complete nucleotide sequence and analysis of the putative polyprotein of maize dwarf mosaic virus genomic RNA (Bulgarian isolate). Archives of Virology 143: 1791–1799. DOI: https://doi.org/10.1007/s00705....
15.
Li X., Hu W., Li Y., Li Y., Chen S., Wang J. 2022. Development of an RT-LAMP assay for the detection of maize yellow mosaic virus in maize. Journal of Virology Methods 300: 114384. DOI: https://doi.org/10.1016/j.viro....
16.
Panno S., Matić S., Tiberini A., Caruso A.G., Bella P., Torta L., Stassi R., Davino A.S. 2020. Loop mediated isothermal amplification: principles and applications in plant virology. Plants (Basel) 9 (4): 461. DOI: https://doi.org/10.3390/plants....
17.
Rosen S., Skaletski H.J. 2000. p. 365–386. Primer3 on the WWW for general use and for biologist programmers. In: “Bioinformatics Methods and Protocols: Method in Molecular Biology” (S. Krawetz, S. Misenes, eds.). Humana Press, Totowa/New Jersey. DOI: https://doi.org/10.1385/1-5925....
18.
Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W In: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choise. Nucleic Acids Research 22: 4673–4680. DOI: https://doi.org/10.1093/nar/22....
19.
Trzmiel K. 2008. Molecular characterization of distinct Maize dwarf mosaic virus (MDMV) isolates occuring on maize in Poland. Acta Biochimica Polonica 55 Suppl. 3/2008: 159.
20.
Trzmiel K. 2009. Detection of the Polish isolate of Maize dwarf mosaic virus (MDMV) using a real-time RT-PCR assay. Acta Biochimica Polonica 56 Suppl. 3/2009: 30.
21.
Trzmiel K., Jeżewska M. 2008. Identification of Maize dwarf mosaic virus in maize in Poland. Plant Disease 92 (6): 981. DOI: https://doi.org/10.1094/PDIS-9....
22.
Wijayasekara D., Ali A. 2021. Evolutionary study of maize dwarf mosaic virus nearly complete genome sequences acquired by next-generation sequencing. Scientific Reports 11: 18786. DOI: https://doi.org/10.1038/s41598....
23.
Yao M.C., Zhu P.X., Zhang Q.Q., Zhang Q.P., Ji Z.L., Zhu F. 2022. Development of a reverse transcription-loop mediated isothermal amplification assay for detection of wisteria vein mosaic virus European Journal of Plant Pathology 163: 13–123. DOI: https://doi org/10.1007/s10658-022-02460-7.
24.
Zarzyńska-Nowak A., Hasiów-Jaroszewska B., Jeżewska M. 2018. Molecular analysis of barley stripe mosaic virus isolates differing in their biological properties and the development of reverse transcription loop-mediated isothermal amplification assays for their detection. Archives of Virology 163 (5): 1163–1170. DOI: https://doi.org/10.1007/s00705....
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| ISSN: | 1427-4345 |
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