ORIGINAL ARTICLE
Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum
1
Brazilian Agricultural Research Corporation-Embrapa Environment, C.P.69, 13820-000, Jaguariúna-SP, Brazil
2
Departament of Biology, Faculdade de Engenharia, UNESP, C.P.31, 15385-000, Ilha Solteira-SP, Brazil
3
School of Biological Sciences, Birkbeck College, Malet Street, London WC1E 7HX, London, UK
Corresponding author
Itamar S. Melo
Brazilian Agricultural Research Corporation-Embrapa Environment, C.P.69, 13820-000, Jaguariúna-SP, Brazil
Brazilian Agricultural Research Corporation-Embrapa Environment, C.P.69, 13820-000, Jaguariúna-SP, Brazil
Journal of Plant Protection Research 2011;51(2):179-183
KEYWORDS
Coniothyrium minitansSclerotinia sclerotiorumiprodioneresistancebiological controlantibiotic production
TOPICS
ABSTRACT
Coniothyrium
minitans
(CM) is hyperparasitic to
Sclerotinia sclerotiorum
(SS), a pathogen of many economically important
crops. In this paper, we describe the isolation of improved mutants of CM, using a UV – irradiation regime, with altered chitinase
production and tolerance to high concentration of iprodione, which are effective against SS. Three out of the 59 mutants obtained
inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80% of sclerotia were colonized by the mutants and the wild-type CM. The mutant strains retained
ability to produce significant amounts of chitinase. The mutants differed from their wild-type strain in appearance, morphology and
sporulation. In conclusion, the results presented here provide evidence that the new biotypes of
C. minitans
are effective in controlling
S. sclerotiorum
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| ISSN: | 1427-4345 |
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