ORIGINAL ARTICLE
Detoxifying enzyme studies on cotton leafhopper, Amrasca biguttula biguttula (Ishida), resistance to neonicotinoid insecticides in field populations in Karnataka, India
1
Department of Agricultural Entomology, University of Agricultural Sciences, Dharwad, 580-005, Karnataka, India
Submission date: 2016-03-30
Acceptance date: 2016-10-10
Corresponding author
Banakar Halappa
Department of Agricultural Entomology, University of Agricultural Sciences, Dharwad, 580-005, Karnataka, India
Department of Agricultural Entomology, University of Agricultural Sciences, Dharwad, 580-005, Karnataka, India
Journal of Plant Protection Research 2016;56(4):346-352
KEYWORDS
TOPICS
ABSTRACT
The cotton leafhopper (Amrasca biguttula biguttula Ishida) is considered to be an alarming insect pest causing both quantitative and qualitative loss in cotton. In situ bioassay studies were done and the role of detoxifying enzymes in conferring resistance to neonicotinoid groups of insecticides in low (MUD), medium (DVG), high (HVR) and very high (GLB) pesticide usage areas of Karnataka were determined. Bioassay studies showed that imidacloprid, thiamethoxam, acetamiprid, thiacloprid and clothianidin registered varying levels of resistance for all the locations studied. The resistance ratio was high in imidacloprid (3.35, 8.57, 9.15 and 12.27 fold respectively) and the lowest in dinoferuran (1.86, 5.13, 6.71 and 9.88 fold respectively). Furthermore, the enzyme activity ratio (glutathione-S-transferase) was relatively greater, and corresponded to the higher LC 50 values of neonicotinoids for very high, high, medium and low pesticide usage areas. Our study suggested that the higher activity of the detoxifying enzyme in the resistance population of cotton leafhopper apparently has a significant role in endowing resistance to neonicotinoid groups of insecticides.
However, this study recommends using neonicotinoids in cotton growing areas with caution.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (48)
1.
Abbott W.S. 1925. A method for computing the effectiveness of an insecticide. Journal of Economic Entomology 18 (2): 265–267.
2.
Anonymous 2013. Annual Report 2011–12. Central Institute for Cotton Research. Nagpur, Maharashtra, India, p. 210–213.
3.
Anonymous 2015. Annual Report 2014–15. All India Coordinated Cotton Improvement Project. Coimbatore, India, p. 217–218.
4.
Bai D., Lummis S.C.R., Leicht W., Breer H., Sattelle D.B. 1991. Actions of imidacloprid and a related nitromethylene on cholinergic receptors of an identified insect motor neurone. Pesticide Science 33 (2): 197–204.
5.
Benedict J.H. 1996. Bt cotton: Opportunities and challenges. p. 25–29. In: Proceedings of the Beltwide Cotton Conference Nashville, National Cotton Council. Am., Memphis, 9–12 January 1996.
6.
Bennett R.M., Ismael Y., Kambhampati U., Morse S. 2004. Economic impact of genetically modified cotton in India. Agriculture Biotechnology Forum 7 (3): 96–100.
7.
Chalam M.S.V., Subbaratnam G.V. 1999. Insecticide resistance in cotton leafhopper. Amrasca biguttula biguttula (Ishida) in Andhra Pradesh. Pest Management and Economic Zoology 7 (2): 105–110.
8.
Chalam M.S.V., Subbaratnam G.V., Rao G.R.C. 2001. Role of mixed function oxidases in imparting resistance to the cotton leafhopper, Amrasca biguttula biguttula (Ishida). Pest Management and Economic Zoology 9 (1): 49–53.
9.
Chao S.L., Dennehy T.J., Casida J.E. 1997. Whitefly (Hemiptera: Aleyrodidae) binding site for imidacloprid and related insecticides: a putative nicotinic acetylcholine receptor. Journal of Economic Entomology 90 (4): 879–882.
10.
Clark A.G., Shamaan N.A. 1984. Evidence that DDT-dehydrochlorinase from the house fly is a glutathione S-transferase. Pesticide Biochemistry and Physiology 22 (3): 249–261.
11.
Dhaliwal G.S., Arora R. 2001. Role of phytochemicals in integrated pest management. p. 97–117. In: “Phytochemical Biopesticides” (O. Koul, G.S. Dhaliwal, eds.). Harwood Academic Publishers, Amsterdam, Netherlands, 223 pp.
12.
Dhaliwal G.S., Ram Singh, Chhillar B.S. 2006. Essentials of Agricultural Entomology. Kalyani Publishers, Ludhiana, India, 451 pp.
13.
Dhawan A.K., Sidhu A.S., Simwat G.S. 1988. Assessment of avoidable loss in cotton (Gossypium hirsutum and G. arboreum) due to sucking pests and bollworms. Indian Journal of Agricultural Sciences 58 (4): 290–292.
14.
Gao B., Wu J., Huang S., Mu L., Han Z. 2008. Insecticide resistance in field populations of Laodelphax striatellus Fallén (Homoptera: Delphacidae) in China and its possible mechanisms. International Journal of Pest Management 54 (1): 13–19.
15.
Habig W.H., Pabst J., Jakoby W.B. 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry 249 (22): 7130–7139.
16.
Hargreaves H. 1948. List of Recorded Cotton Insects of the World. Commonwealth Institute of Entomology, London, UK, 50 pp.
17.
Hayes J.D., Wolf C.R. 1988. Role of glutathione transferase in drug resistance. p. 315–355. In: “Glutathione Conjugation: Mechanisms and Biological Significance” (H. Sies, B. Ketterer), Academic Press, London, UK, 480 pp.
18.
Hayes J.D., Pulford D.J. 1995. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Critical Review in Biochemistry and Molecular Biology 30 (6): 445–600.
19.
Hemingway J., Miyamoto J., Herath P.R.J. 1991. A possible novel link between organophosphorus and DDT insecticide resistance genes in Anopheles: supporting evidence from fenitrothion metabolism studies. Pesticide Biochemistry and Physiology 39 (1): 49–56.
20.
Hong-Bin Z., Yaning L., Baohua W., Peng W.C. 2008. Recent advances in cotton genomics. International Journal of Plant Genomics 2008: 1–20.
21.
Horowitz A.R., Kontsedalov S., Ishaaya I. 2004. Dynamics of resistance to the neonicotinoids, acetamiprid and thiamethaxam in Bemisia tabaci (Homoptera: Aleyrodidae). Journal of Economic Entomology 97 (6): 2051–2056.
22.
Abdallah I.S., Abou-Yousef H.M., Fouad E.A., Kandil M.A. 2016. The role of detoxifying enzymes in the resistance of the cowpea aphid (Aphis craccivora Koch) to thiamethoxam. Journal of Plant Protection Research 56 (1): 67–72.
23.
Jakoby W.B. 1978. The glutathion-S-transferase: a group of multifunctional detoxification proteins. Advances in Enzymology and Related Areas of Molecular Biology 46: 383–414.
24.
Jenkins J.N., McCarty J.C., Buehler R.E., Kiser J., Williams C., Wofford T. 1997. Resistance of cotton with δ-endotoxin genes from Bacillus thuringiensis var. kurstaki on selected lepidopteran insects. Agronomy Journal 89 (5): 768–780.
25.
Kohel R.J., Yu J., Park Y.H., Lazo G.R. 2001. Molecular mapping and characterization of traits controlling fiber quality in cotton. Euphytica 121 (2): 163–172.
26.
Kranthi K.R. 2007. Insecticide resistance management in cotton to enhance productivity. p. 214–231. In: “Model Training Course on Cultivation of Long Staple Cotton (ELS)”. Central Institute for Cotton Research Regional Station, Coimbatore, Tamil Nadu, India, 15–22 December 2007.
27.
Kshirsagar S.D., Satpute N.S., Moharil M.P. 2012. Monitoring of insecticide resistance in cotton leafhoppers, Amrasca biguttula biguttula (Ishida). Annals of Plant Protection Sciences 20 (2): 283–286.
28.
Liu M.Y., Lanford J., Casida J.E. 1993. Relevance of [3H] imidaclorid binding site in house fly head acetylcholine receptor to insecticidal activity of 2-nitromethylene- and 2-nitroiminoimidazolidines. Pesticide Biochemistry and Physiology 46 (3): 200–206.
29.
Mayee C.D., Gautam H.C., Barik A. 2004. Cotton scenario in India vis-à-vis world and future need. p. 245–253. In: “Recent Advances in Cotton Research and Development”(M.S. Chauhan, R.K. Sain, eds.), Haryana Agricultural University and Cotton Research and Development Association, CCSHAU, Hisar.
30.
McAuslane H.J., Johnson F.A., Knauft D.A., Colvin D.L. 1993. Seasonal abundance and within-plant distribution of parasitoids of Bemisia tabaci (Homoptera: Aleyrodidae) in peanuts. Environment Entomology 22 (5): 1043–1050.
31.
Nibouche S. 1994. Evolutionary cycle of Helicoverpa armigera (Hübner) in western regions of Burkina Faso. Ph.D. thesis, National Institute of Higher Education in Agricultural Sciences, Montpellier, France, 210 pp.
32.
Painter R.H. 1951. Insect Resistance in Crop Plants. The Macmillan Company, New York, USA, 520 pp.
33.
Pandi R. 1997. Ecology and Management of Leafhopper. Amrasca devastans (Distant) in Cotton. M.Sc. thesis, Tamil Nadu Agricultural University, Coimbatore, India, 143 pp.
34.
Perlak F.J., Oppenhuizen M., Gustafson K., Voth R., Sivasupramaniam S., Heering D., Carey B., Ihrig R.A., Roberts J.K. 2001. Development and commercial use of Bollgard® cotton in the USA – early promises versus today’s reality. The Plant Journal 27 (6): 489–501.
35.
Praveen P.M. 2003. Studies on insecticide resistance in early season sucking pests of cotton in Tamil Nadu. Ph.D. thesis, Tamil Nadu Agricultural University, Coimbatore, India, 193 pp.
36.
Preetha G., Stanley J., Manoharan T., Kuttalam S. 2013. Baseline toxicity of chloronicotinyls against cotton leafhopper, Amrasca biguttula biguttula, and detection of insecticide resistance. Archives of Phytopathology and Plant Protection 47 (17): 2095–2105.
37.
Regupathy A., Ayyasamy R. 2004. Insecticide resistance in sucking pests of cotton and resurgence. p. 42. In: Proceedings of the International Symposium on Strategies for Sustainable Cotton Production: A Global Vision. Crop Protection. University of Agricultural Sciences, Dharwad, Karnataka, India, 23–25 November 2004, 231 pp.
38.
Rohini A., Prasad N.V.V.S.D., Chalam M.S.V. 2012. Management of major sucking pests in cotton by insecticides. Journal of Cotton Research and Development 20 (1): 102–106.
39.
Sagar D., Balikai R.A., Khadi B.M. 2013. Insecticide resistance in leafhopper, Amrasca biguttula biguttula (Ishida) of major cotton growing districts of Karnataka, India. Biochemical and Cellular Archives 13 (2): 261–265.
40.
Santhini S., Uthamasamy S. 1998. Susceptibility of cotton leaf-hopper (Amrasca devastans) to insecticides in Tamil Nadu. The Indian Journal of Agriculture Science 68 (3): 184–185.
41.
Singh R., Agarwal R.A. 1988. Role of chemical components of resistant and susceptible genotypes of cotton and okra in ovipositional preference of cotton leafhopper. Proceedings: Animal Sciences 97 (6): 545–550.
42.
Stansly P.A., Schuster D.J., Liu T.X. 1997. Apparent parasitism of Bemisia argentifolii (Homoptera: Aleyrodidae) by Aphelinidae (Hymenoptera) on vegetable crops and associated weeds in South Florida. Biological Control 9 (1): 49–57.
43.
Uthamasamy S. 1985. Influence of leaf hairiness on the resistance of bhendi or lady’s finger, Abelmoschus esculentus (L.) Moench, to the leaf hopper, Amrasca devastans (Dist.). Tropical Pest Management 31 (4): 194–295.
44.
Wang K.Y., Liu T.X., Jiang X.Y., Yi M.Q. 2001. Cross-resistance of Aphis gossypii to selected insecticides on cotton and cucumber. Phytoparasitica 29 (5): 393–399.
45.
Wen Y., Liu Z., Bao H., Han Z. 2009. Imidacloprid resistance and its managements in field populations of brown planthopper, Nilparvata lugens Stål in China. Pesticide Biochemistry and Physiology 94 (1): 36–42.
46.
Xie X.H. 1998. A new type of insecticide – imidacloprid. Agrochemicals 37: 40–42. (in China).
47.
Yang J.C., Li M., Chai B.S., Liu C.L. 2007. [Recent research advances in new neonicotinoid insecticides]. Agrochemicals 46 (7): 433–438. (in China).
48.
Zhang Y.Y., Zhang H. 1999. The possible occurrence and management of resistance to imidachloprid. Agrochemicals 38: 22–23. (in China).
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
