ORIGINAL ARTICLE
Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays
1
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Submission date: 2017-09-12
Acceptance date: 2017-08-17
Corresponding author
Khosrow Chehri
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Journal of Plant Protection Research 2017;57(3):237-242
KEYWORDS
TOPICS
ABSTRACT
In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members of FGSC was confirmed molecularly using Fg16F/Fg16R primers. Fusarium asiaticum isolates (4) were distinguished from other FGSC using Fg6CTPSf177/Fg16R primers. Polymerase chain reaction-based (PCRbased) detection of mycotoxin-synthesis-pathway gene was also used to determine the potential of the analysed strains to produce deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-AcDON), 3-acetyldeoxynivalenol (3-AcDON), and nivalenol (NIV). Of 14 tested isolates, 10 and 4 isolates belonged to DON and NIV chemotype, respectively. Also, the results of DON chemotype survey using specific primers MinusTri7F/R and Tri315F/R showed 1 and 9 isolates produced 3-AcDON and 15-AcDON, respectively. These results show that DON was the most common chemotype in western Iran. To our knowledge, this is the first report on 15-AcDON, 3-AcDON, and NIV isolated from barley in Iran.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (33)
1.
Akinsanmi O.A., Mitter V., Simpfendorfer S., Backhouse D., Chakraborty S. 2004. Identity and pathogenicity of Fusarium spp. isolated from wheat fields in Queensland and northern New South Wales. Australian Journal of Agricultural Research 55 (1): 97–107. DOI: https://doi.org/10.1071/ar0309....
2.
Backhouse D. 2014. Global distribution of Fusarium graminearum, F. asiaticum and F. boothii from wheat in relation to climate. European Journal of Plant Pathology 139 (1): 161–173. DOI: https://doi.org/10.1007/s10658....
3.
Boutigny A.L., Ward T.J., Van Coller G.J., Flett B., Lamprecht S.C., O’Donnell K., Viljoen A. 2011. Analysis of the Fusarium graminearum species complex from wheat, barley and maize in South Africa provides evidence of species-specific differences in host preference. Fungal Genetics and Biology 48 (9): 914–920.
4.
Chandler E.A., Duncan R.S., Thomsett M.A., Nicholson P. 2003. Development of PCR assays to tri7 and tri13 and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis. Physiological and Molecular Plant Pathology 62 (6): 355–367. DOI: https://doi.org/10.1016/s0885-....
5.
Davari M., van Diepeningen A.D., Babai-Ahari A., Arzanlou M., Najafzadeh M.J., van der Lee T.A., de Hoog G.S. 2012. Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA). Journal of Microbiological Methods 89 (1): 63–70. DOI: https://doi.org/10.1016/j.mime....
6.
Goswami R.S., Kistler H.C. 2004. Heading for disaster: Fusarium graminearum on cereal crops. Molecular Plant Pathology 5 (6): 515–525. DOI: https://doi.org/10.1111/j.1364....
7.
Haratian M., Sharifnabi B., Alizadeh A., Safaie N. 2008. PCR analysis of the Tri13 gene to determine the genetic potential of Fusarium graminearum isolates from Iran to produce nivalenol and deoxynivalenol. Mycopathologia 166 (2): 109–116. DOI: https://doi.org/10.1007/s11046....
8.
Jennings P., Coates M.E., Turner J.A., Chandler E.A., Nicholson P. 2004. Determination of deoxynivalenol and nivalenol chemotypes of Fusarium culmorum isolates from England and Wales by PCR assay. Plant Pathology 53 (2): 182–190. DOI: https://doi.org/10.1111/j.0032....
9.
Karugia G.W., Suga H., Gale L.R., Nakajima T., Tomimura K., Hyakumachi M. 2009. Population structure of the Fusarium graminearum species complex from a single Japanese wheat field sampled in two consecutive years. Plant Disease 93 (2): 170–174. DOI: https://doi.org/10.1094/pdis-9....
10.
Kim J-C., Kang H-J., Lee D.H., Lee Y.W., Yoshizawa T. 1993. Natural occurrence of Fusarium mycotoxins (trichothecenes and zearalenone) in barley and maize in Korea. Applied and Environmental Microbiology 59 (11): 3798–3802.
11.
Lee J., Chang I.Y., Kim H., Yun S.H., Leslie J.F., Lee Y.W. 2009. Genetic diversity and fitness of Fusarium graminearum populations from rice in Korea. Applied and Environmental Microbiology 75 (10): 3289–3295. DOI: https://doi.org/10.1128/aem.02....
12.
Lenart A.M., Klimek-Kopyra A., Boroń P.M. 2013. Morphological and molecular identification and PCR amplification to determine the toxigenic potential of Fusarium spp. isolated from maize ears in southern Poland. Phytoparasitica 41 (3): 241–248. DOI: https://doi.org/10.1007/s12600....
13.
Lenc L., Lukanowski A., Sadowski C. 2008. The use of PCR amplification in determining the toxigenic potential of Fusarium sambucinum and F. solani isolated from potato tubers with symptoms of dry rot. Phytopathologia Polonica 48: 12–23.
14.
Leslie J.F., Summerell B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publish Ltd., UK, 388 pp.
15.
Li H.P., Wu A.B., Zhao C.S., Scholten O., Lőffler H., Liao Y.C. 2005. Development of a generic PCR detection of deoxynivalenol and nivalenol chemotypes of Fusarium graminearum. FEMS Microbiology Letters 243 (2): 505–511. DOI: https://doi.org/10.1016/j.fems....
16.
McMullen M., Jones R., Gallenberg D. 1997. Scab of wheat and barley: a reemerging disease of devastating impact. Plant Disease 81 (12): 1340–1348. DOI: https://doi.org/10.1094/pdis.1....
17.
Miller J.D., Greenalgh R., Wang Y., Lu M. 1991. Trichothecene chemotype of three Fusarium species. Mycologia 83 (2): 121–130. DOI: https://doi.org/10.2307/375992....
18.
Nash S.M., Snyder W.C. 1962. Quantitative estimations by plat counts of propagules of the Bean rot rot Fusarium in field soils. Phytopathology 52 (6): 567–572.
19.
Nicholson P., Simpson D.R., Weston G., Rezanoor H.N., Lees A.K., Parry D.W., Joyce D. 1998. Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology 53 (1): 17–37. DOI: https://doi.org/10.1006/pmpp.1....
20.
Obanor F., Neate S., Simpfendorfer S., Sabburg R., Wilson P., Chakraborty S. 2013. Fusarium graminearum and Fusarium pseudograminearum caused the 2010 head blight epidemics in Australia. Plant Pathology 62 (1): 79–91. DOI: https://doi.org/10.1111/j.1365....
21.
Postic J., Cosic J., Vrandecic K., Jurkovic D., Saleh A.A., Leslie J.F. 2012. Diversity of Fusarium species isolated from weeds and plant debris in Croatia. Journal of Phytopathology 160 (2): 76–81. DOI: https://doi.org/10.1111/j.1439....
22.
Quarta A., Mita G., Haidukowski M., Santino A., Mulè G., Visconti A. 2006. Assessment of trichothecene chemotypes of Fusarium culmorum occurring in Europe. Food Additives and Contaminants 22 (4): 309–315. DOI: https://doi.org/10.1080/026520....
23.
Qu B., Li H., Zhang J., Xu Y., Huang T., Wu A., Zhao C., Carter J., Nicholson P., Liao Y. 2008. Geographic distribution and genetic diversity of Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathology 57 (1): 15–24. DOI: https://doi.org/10.1111/j.1365....
24.
Sampietro D.A., Ficoseco M.E.A., Jimenez C.M., Vattuone M.A., Catalán CA. 2012. Trichothecene genotypes and chemotypes in Fusarium graminearum complex strains isolated from maize fields of northwest Argentina. International Journal of Food Microbiology 153 (1–2): 229–233. DOI: https://doi.org/10.1016/j.ijfo....
25.
Sampietro D.A., Marin P., Iglesias J., Presello D.A., Vattuone M.A., Catalan C.A.N. 2010. A molecular based strategy for rapid diagnosis of toxigenic Fusarium species associated to cereal grains from Argentina. Fungal Biology 114 (1): 74–81. DOI: https://doi.org/10.1016/j.mycr....
26.
Sarver B., Ward T., Gale L., Broz K., Kistler H.C., Aoki T., Nicholson P., Carter J., O’Donnell K. 2011. Novel Fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genealogical concordance. Fungal Genetic and Biology 48 (12): 1096–1107. DOI: https://doi.org/10.1016/j.fgb.....
27.
Somma S., Petruzzella A.L., Logrieco A.F., Meca G., Cacciola O.S., Moretti A. 2014. Phylogenetic analyses of Fusarium graminearum strains from cereals in Italy, and characterisation of their molecular and chemical chemotypes. Crop and Pasture Science 65 (1): 52–60. DOI: https://doi.org/10.1071/cp1331....
28.
Waalwijk C., Kastelein P., Vries I. 2003. Major changes in Fusarium spp. in wheat in the Netherlands. European Journal of Plant Pathology 109: 743–754. DOI: https://doi.org/10.1007/978-94....
29.
Ward T.J., Bielawski J.P., Kistler H.C., Sullivan E., O’Donnell K. 2002. Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytopathogenic Fusarium. Proceedings of the National Academy of Sciences, USA 99 (14): 9278–9283. DOI: https://doi.org/10.1073/pnas.1....
30.
Yang L., van der Lee T., Yang X.D., Waalwijk C. 2008. Fusarium populations on Chinese barley show a dramatic gradient in mycotoxin Profile. Phytopathology 98 (6): 719–722. DOI: https://doi.org/10.1094/phyto-....
31.
Yoruk E., Tunali B., Kansu B., Olmez F., Uz G., Zumrut I.M., Sarıkaya A., Meyva G. 2016. Characterization of high-level deoxynivalenol producer Fusarium graminearum and F. culmorum isolates caused head blight and crown rot diseases in Turkey. Journal of Plant Diseases and Protection 123: 177–186. DOI: https://doi.org/10.1007/s41348....
32.
Zhang H., van der Lee T., Waalwijk C., Chen W., Xu J., Jin Xu J.S., Zheng Y., Feng J. 2012. Population analysis of the Fusarium graminearum species complex from wheat in China show a shift to more aggressive isolates. PLoS One 7 (2): e31722. DOI: https://doi.org/10.1371/journa....
33.
Zhang J.B., Li H.P., Dang F.J., Qu B., Xu Y.B., Zhao C.S., Liao Yu-C. 2007. Determination of the trichothcene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of the Fusarium graminearum clade from China. Mycological Research 111 (8): 967–975. DOI: https://doi.org/10.1016/j.mycr....
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
