ORIGINAL ARTICLE
Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran
1
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural
Resources, University of Tehran, Karaj,
31587-77871,
Iran
Submission date: 2015-01-28
Acceptance date: 2015-06-26
Corresponding author
Akbar Dizadji
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
Journal of Plant Protection Research 2015;55(3):235-240
KEYWORDS
TOPICS
ABSTRACT
Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To
assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed
on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3’ end of one
mechanically non-transmitted potyvirus isolate, comprising a 3’ untranslated region (390 bp) and C-terminus of the coat protein (CP)
gene (459 bp), was amplified by reverse transcription polymerase chain reaction (RT-PCR), which was ligated into pTG19-T vector.
The nucleotide sequence of amplicons
was compared with related sequences, using Blastn software available at NCBI GenBank, and
showed the highest similarity with
Iris severe mosaic virus
(ISMV) isolates. The nucleotide and deduced amino acid sequence of the CP
C-terminus region was more than 83% identical with other ISMV isolates, therefore this isolate was designated as ISMV-Ir. This new
ISMV isolate is closely related to the Chinese ISMV-PHz in phylogenetic analysis, based on the partial nucleotide and deduced amino
acid sequence of the CP region. This is the first report of ISMV occurrence on
Iris
spp. in Iran
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| ISSN: | 1427-4345 |
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