Pesticide spraying robots have the capacity to carry substantial storage reservoirs, ensuring safe and potentially autonomous operation, all at a significantly lower cost than traditional methods. Our study aimed to manufacture and evaluate a prototype autonomous sprayer robot (ASR) for future technologies in plant protection that include a traction system, a spraying mechanism, a Wi-Fi controller for directing the robot's movement, and plant detection with an infrared sensor (IR) as well as a microswitch with a push arm. The developed ASR was evaluated under laboratory conditions using four nozzles (Tee Jet Turbo 110-05, LECHLER 110-04, LECHLER 110-03, and LECHLER 120-015), two travel speeds (0.23 meter/second (m/s) and 0.28 m/s), two spraying heights (50 and 60 cm), and three operating pressures (100, 180, and 250 kPa). Also, the traction system was tested in a straight pass of 10 m to reduce the test error. The results showed that the nozzle type Tee Jet Turbo 110-05 (A) had the highest flow rate at different operating parameters. Additionally, we observed a direct relationship between operating pressure and flow rate, while the flow rate showed an inverse relationship with spraying height across different nozzle types. The coverage percentages were determined in both horizontal and vertical positions. The highest coverage percentage was observed at a spray height of 60 cm and a travel speed of 0.23 m/s. Finally, using a developed control system that contains a microswitch and push arm led to a decrease in volumetric application rate mode by about 31.45% to 55.21% compared to continuous flow rate mode.