ORIGINAL ARTICLE
DNA insecticides: The effect of concentration on non-target plant organisms such as wheat (Triticum aestivum L.)
1
Department of Agrobiotechnology, People’s Friendship University of Russia, 117 198, Moscow, Russia
2
Department of Biochemistry, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Republic of Crimea
3
Department of Plant Physiology and Biotechnology, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Republic of Crimea
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-09-16
Acceptance date: 2018-02-26
Online publication date: 2019-03-25
Corresponding author
Palmah Mutah Nyadar
Department of Agrobiotechnology, People’s Friendship University of Russia, 117 198, Moscow, Russia
Department of Agrobiotechnology, People’s Friendship University of Russia, 117 198, Moscow, Russia
Journal of Plant Protection Research 2019;59(1):60-68
KEYWORDS
TOPICS
ABSTRACT
The excessive use of pesticides is a problem in most parts of the world today because of
their broad and unspecific target range that is considerably harmful. The accumulation of
several chemical insecticide residues based on chlorpyrifos-methyl, organochlorine, different
isomers of HCH, DDT etc., in Triticum aestivum L. plants can be dangerous. Hence,
there is an urgent need to develop potential and safer alternative measures. Wheat (Triticum aestivum L.) is a major cereal crop grown and used for food, animal feed, beverages
and furniture accessories in most parts of the world. It also serves as a host to various
insect pests. Our previous studies showed the insecticidal potency and specificity of short
ssDNA oligonucleotides from the inhibitor of apoptosis (IAP-2 and IAP-3) genes of Lymantria
dispar multicapsid nuclear polyhedrosis virus (LdMNPV) against gypsy moth (L. dispar)
larvae, a possible insect pest of non-host plants like wheat. Consequently, the present
study analyzes the effects of ssDNA oligonucleotides used as DNA insecticides on wheat (T. aestivum) plant biomass, plant organs and some biochemical parameters as a marker of
the safety margin on non-target organisms. The results obtained on plant biomass showed
that groups treated with ssDNA oligonucleotides at concentrations of 0.01 pmol · μl−1,
0.1 pmol · μl−1 and 1 pmol · μl−1 varied in comparison with the control group, but remained
harmless to plant growth and development, while the treatment concentration of
0.001 pmol · μl−1 did not affect the plant biomass. The glucose, protein and phosphorous
biochemical parameters, analyzed after 21 days, showed that the ssDNA oligonucleotides
used were equally safe. The data obtained for the plant organs (leaves and root lengths)
indicate that the phenomenon of DNA insecticides can be further studied and developed
for plant protection while improving the growth of plant organs even for a non-target
organism such as wheat T. aestivum plants.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (38)
1.
Aktar W., Dwaipayan S., Ashim C. 2009. Impact of pesticides use in agriculture: their benefits and hazards. Interdisciplinary toxicology 2 (1): 1−12. DOI: 10.2478/v10102-009-0001-7.
2.
Bashline L., Lei L., Li S., Gu Y. 2014. Cell wall, cytoskeleton, and cell expansion in higher plants. Molecular Plant 7 (4): 586–600. DOI: 10.1093/mp/ssu018.
3.
Bragança I., Lemos P.C., Barros P., Delerue-Matos C., Domingues V.F. 2018. Phytotoxicity of pyrethroid pesticides and its metabolite towards Cucumis sativus. Science of the Total Environment 619: 685−691. DOI: 10.1016/j.scitotenv.2017.11.164.
4.
Burger J., Mol F., Gerowitt B. 2008. The ‘necessary extent’ of pesticide use − Thoughts about a key term in German pesticide policy. Crop Protection 27: 343–351. DOI: 10.1016/j.cropro.2007.06.006.
5.
Chauhan S.S., Agrawal S.A., Srivastava A.N. 2013. Effect of imidacloprid insecticide residue on biochemical parameters in potatoes and its estimation by HPLC. Asian Journal of Pharmaceutical and Clinical Research 6 (3): 114−117.
6.
Cosgrove D.J. 2000. Expansive growth of plant cell walls. Plant Physiology and Biochemistry 38 (1): 109−124. DOI: 10.1016/S0981-9428(00)00164-9.
7.
Da Silva L.C., Correia M.T. 2014. Plant lectins and Toll-like receptors: implications for therapy of microbial infections. Frontiers in Microbiology 5: 20. DOI: 10.3389/fmicb.2014.00020.
8.
Ecobichon D.J. 2001. Pesticide use in developing countries. Toxicology 160 (1): 27−33.
9.
Eleftherohorinos I.G. 2008. Weed Science: Weeds, Herbicides, Environment, and Methods for Weed Management. Agro-Typos, Athens, Greece, 408 pp.
10.
EPA (United States Environmental Protection Agency). 2009. Registering Pesticides. http://www.epa.gov/pesticides/....
11.
Farcas A., Matei A.V., Florian C., Badea M., Coman G. 2013. Health effects associated with acute and chronic exposure to pesticides. NATO Science for Peace and Security Series C: Environmental Security 134: 103–110. DOI: 10.1007/978-94-007-6461-3_8.
12.
Grewal A.S., Grewal A.S., Singla A., Kamboj P., Dua J.S. 2017. Pesticide residues in food grains, vegetables and fruits: a hazard to human health. Journal of Medicinal Chemistry and Toxicology 2 (1): 40−46. DOI: 10.15436/2575-808-X.17.1355.
13.
Gill R.J., Raine N.E. 2014. Chronic impairment of bumblebee natural foraging behavior induced by sublethal pesticide exposure. Functional Ecology 28 (6): 1459–1471. DOI: https://doi.org/10.1111/1365-2....
14.
Gray J.W., Burns C.J., Mahlburg W.M. 2013. Increased cancer burden among pesticide applicators and others due to pesticide exposure. CA: A Cancer Journal for Clinicians 63 (5): 364–366. DOI: 10.3322/caac.21194.
15.
Hershey D.R. 1998. Bean seed imbibition. Science Activities: Classroom Projects and Curriculum Ideas 35 (2): 25−27. DOI: 10.1080/00368129809602077.
16.
Imler J.L., Zheng L. 2004. Biology of toll receptors: lessons from insects and mammals. Journal Leukocyte Biology 75 (1): 18−26. DOI: 10.1189/jib.0403160.
17.
Kozuka T., Sam-Geun K., Michio D., Ken-ichiro S., Akira N. 2011. Tissue-autonomous promotion of palisade cell development by phototropin 2 in Arabidopsis. The Plant Cell 23 (10): 3684−3695. DOI: 10.1105/tpc.111.085852.
18.
Kuzio J., Pearson M.N., Harwood S.H., Funk C.J., Evans J.T., Slavicek J.M., Rohrmann G.F. 1999. Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar. Virology 253 (1): 17–34. DOI: 10.1006/viro.1998.9469.
19.
Langley R.L., Mort S.A. 2012. Human exposures to pesticides in the United States. Journal of Agromedicine 17 (3): 300−315. DOI: 10.1080/1059924X.2012.688467.
20.
Lekei E.E., Ngowi A.V., London L. 2014. Farmers’ knowledge, practices and injuries associated with pesticide exposure in rural farming villages in Tanzania. BMC Public Health 14 (1): 389. DOI: 10.1186/1471-2458-14-389.
21.
Maroni M., Fanetti A.C., Metruccio F. 2006. Risk assessment and management of occupational exposure to pesticides in agriculture. La Medicina del Lavoro 97 (2): 430–437.
22.
Nicolopoulou-Stamati P., Maipas S., Kotampasi C., Stamatis P., Hens L. 2016. Chemical pesticides and human health: the urgent need for a new concept in agriculture. Frontiers in Public Health 4: 148. DOI: 10.3389/fpubh.2016.00148.
23.
Nyadar P., Oberemok V., Zubarev I. 2018. A small molecule for a big transformation: topical application of a 20-nucleotidelong antisense fragment of the DIAP-2 gene inhibits the development of Drosophila melanogaster female imagos. Archives of Biological Sciences 70 (1): 33−39. DOI: 10.2298/ABS170302023N.
24.
Nyadar P.M., Adeyemi T.A. 2018. DNA insecticides: the lethal potency of LdMNPV IAP-2 gene antisense oligonucleotides in pre-infected gypsy moth (Lymantria dispar L.) larvae. International Journal of Pest Management 64 (2): 173−177. DOI:10.1080/09670874.2017.1359432.
25.
Nyadar P.M., Zaitsev A.S., Adeyemi T.A., Shumskykh M.N., Oberemok V.V. 2016. Biological control of gypsy moth (Lymantria dispar): an RNAi-based approach and a case for DNA insecticides. Archives of Biological Science 68 (3): 677−683. DOI: 10.2298/ABS150828041N.
26.
Oberemok V., Nyadar P., Zaitsev A., Levchenko N., Shiyntum H., Omelchenko O. 2013. Pioneer evaluation of the possible side effects of the DNA insecticides on wheat (Triticum aestivum L.). International Journal of Biochemistry and Biophysics 1 (3): 57−63. DOI: 10.13189/ijbb.2013.010302.
27.
Oberemok V.V., Nyadar P.M. 2015. Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP-3 gene. Turkish Journal of Biology 39 (2): 258−264. DOI: 10.3906/biy-1406-56.
28.
Oberemok V.V., Laikova K.V., Zaitsev A.S., Shumskykh M.N., Kasich I.N., Gal’chinsky N.V., Bekirova V.V., Makarov V.V., Agranovsky A.A., Gushchin V.A., Zubarev I.V. 2017. Molecular alliance of Lymantria dispar multiple nucleopolyhedrovirus and a short unmodified antisense oligonucleotide of its anti-apoptotic IAP-3 gene: a novel approach for gypsy moth control. International Journal of Molecular Sciences 18 (11): 2446. DOI: 10.3390/ijms18112446.
29.
Pimentel D. 2005. Environmental and economic costs of the application of pesticides primarily in the United States. Environment, Development and Sustainability 7: 229−252. DOI: 10.1007/s10668-005-7314-2.
30.
Riah W., Laval K., Laroche-Ajzenberg E., Mougin C., Latour X., Trinsoutrot-Gattin I. 2014. Effects of pesticides on soil enzymes: A review. Environmental Chemistry Letters 12 (2): 257–273. DOI: 10.1007/s10311-014-0458-2.
31.
Roberts D., Pedmale U.V., Morrow J., Sachdev S., Lechner E., Tang X., Zheng N., Hannink M., Genschik P., Liscum E. 2011. Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3NPH3. The Plant Cell 23 (10): 3627−3640. DOI 10.1105/tpc.111.087999.
32.
Sarwar M. 2015. The dangers of pesticides associated with public health and preventing of the risks. International Journal of Bioinformatics and Biomedical Engineering 1 (2): 130–136.
33.
Schachtman D.P., Robert J.R., Sarah M.A. 1998. Phosphorus uptake by plants: from soil to cell. Plant Physiology 116 (2): 447−453. DOI: 10.1104/pp.116.2.447.
34.
Shakir S.K., Kanwal M., Murad W., Zia ur Rehman Z., Zia ur Rehman S., Daud M.K., Azizullah A. 2016. Effect of some commonly used pesticides on seed germination, biomass production and photosynthetic pigments in tomato (Lycopersicon esculentum). Ecotoxicology 25 (2): 329−341. DOI: 1007/s10646-015-1591-9.
35.
Sheen J. 2014. Master regulators in plant glucose signaling networks. Journal of Plant Biology 57 (2): 67−79. DOI: 10.1007/s12374-014-0902-7.
36.
Soares W.L., Porto M.F. 2009. Estimating the social cost of pesticide use: An assessment from acute poisoning in Brazil. Ecological Economics 68: 2721−2728. DOI: https://doi.org/10.1016/j.ecol....
37.
Wilson C., Tisdell C. 2001. Why farmers continue to use pesticides despite environmental, health and sustainability costs. Ecological Economics 39: 449−462. DOI: 10.1016/S0921-8009(01)00238-5.
38.
Yuan H.X., Xiong Y., Guan K.L. 2013. Nutrient sensing, metabolism, and cell growth control. Molecular cell 49 (3): 379−387. DOI: 10.1016/j.molcel.2013.01.019.
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