Silky bentgrass (Apera spica-venti L.) is one of Central Europe's most troublesome monocotyledonous weeds of winter crops. The study aimed to analyze the competitiveness of biotypes of silky bentgrass against winter wheat, depending on the type of soil substrate and nitrogen fertilization. In this research, in a pot experiment during two seasons, we studied the effect of bentgrass plants, of different sensitivity/resistance to herbicides, on winter wheat in an additive model, carried out on sandy or clay soil, either non-fertilized or fertilized with nitrogen. The competitive indices were calculated based on several wheat morphological and yield features. The multivariate analysis was incorporated to interpret the data. As a result, we found that wheat performance is affected by bentgrass competition. No clear effect of soil type and nitrogen fertilization on the competitiveness of A. spica-venti biotypes was demonstrated. Only in one season was the pyroxsulam-resistant biotype competitive to the winter wheat when growing on fertilized clay soil. Further research with varied numbers of winter wheat and bentgrass is advised to assess crop-weed effects further.