ORIGINAL ARTICLE
Comparison of three different techniques for eradication of Apple mosaic virus (ApMV) from hazelnut (Corylus avellana L.)
1
Molecular Biology and Genetics, Mugla Sitki Kocman University, Mugla, Turkey
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2020-07-28
Acceptance date: 2020-10-01
Online publication date: 2021-03-12
Corresponding author
Ergun Kaya
Molecular Biology and Genetics, Mugla Sitki Kocman University, Kotecli Street, 48000, Mugla, Turkey
Molecular Biology and Genetics, Mugla Sitki Kocman University, Kotecli Street, 48000, Mugla, Turkey
Journal of Plant Protection Research 2021;61(1):11-19
KEYWORDS
TOPICS
ABSTRACT
Numerous plant species around the world suffer from the presence of viruses, which especially
in economically important crops, cause irretrievable damage and/or extensive losses.
Many biotechnological approaches have been developed, such as meristem culture, chemotherapy,
thermotherapy or cryotherapy, to eliminate viruses from infected plants. These
have been used alone or in combination. In this work, meristem culture, thermotherapy and
cryotherapy were compared for Apple mosaic virus elimination from hazelnut local cultivar
“Palaz”. The virus-free plant was also confirmed by reverse transcriptase polymerase
chain reaction (RT-PCR) after each treatment and, the best results were obtained by cryotherapy.
A one step freezing technique, droplet vitrification, was used for cryotherapy, and the best
regeneration percentage was 52%. After cryotherapy, virus-free seedlings of hazelnut local
cultivar “Palaz” were confirmed as being virus-free after three subcultured periods.
FUNDING
This study was supported by Mugla Sitki Kocman
University, Scientific Research Projects Coordination
Unit (Mugla, Turkey, MSKU-BAP, Project Number:
17-057).
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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