ORIGINAL ARTICLE
Comparative evaluation of free and bound phenolic acid contents in early grains of durum wheat line for its resistance to fusarium head blight with some other sensitive varieties in Algeria
1
Centre de Recherche en Aménagement du Territoire, CRAT, Campus Universitaire Zouaghi Slimane, Constantine, Algérie
2
Ecole Nationale Supérieure Agronomique, ENSA, El-Harrach, Alger, Algérie
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-05-16
Acceptance date: 2022-07-25
Online publication date: 2022-08-26
Corresponding author
Salah Hadjout
Centre de Recherche en Aménagement du Territoire, CRAT, Campus Universitaire Zouaghi Slimane, Constantine, Algérie
Centre de Recherche en Aménagement du Territoire, CRAT, Campus Universitaire Zouaghi Slimane, Constantine, Algérie
Journal of Plant Protection Research 2022;62(3):287-294
HIGHLIGHTS
- Much higher content of bound phenolic acids compared to free phenolic acids at all genotypes.
- The most contained phenolic acid was ferulic acid followed by p-coumaric acid.
- Predominance of monomeric forms and dimers of ferulic acid (DiFA) in wheat ears.
- Higher concentrations of free and cell wall bound phenolic acids at the flowering stage in the G1 line.
KEYWORDS
TOPICS
ABSTRACT
All plants contain varying levels of phenolic acids (metabolites) thus playing an important
role in resistance mechanisms as constituents of cell walls, as constitutive antimicrobial
compounds of plants or induced in response to infection against many diseases, in particular
fusarium head blight caused by Fusarium species. To this end, the objective of this
research was to study the variation in phenolic acid composition during the kinetics of
filling wheat grains, in order to determine the best variety resistant to fusarium head blight.
For this purpose, free and bound phenolic analyses were carried out by HPLC-DAD on
five durum wheat varieties at the stage 5 to 8 days after the flowering stage (early grains).
We showed that at the level of the samples analyzed, several phenolic acids were present at
different concentrations, but others were absent [cis-ferulic acid (free phenolic acid), and
sinapic acid (bound phenolic acid)]. The results also showed that the content of bound
phenolic acids was much higher than that of free phenolic acids in all varieties. In addition,
these phenolic acids existed in free soluble form or were mostly present in insoluble form
bound to cell walls. For free acids, the results showed that significant amounts of transferulic
acid were detected in comparison to all free phenolic acids (56.72 μg · g–1 DM for
G10). For bound acids, ferulic acid is the main bound phenolic acid which has much higher
levels (4913.92 μg · g–1 DM for G1), followed by p-coumaric acid (3098.99 μg · g–1 DM
for G1). Moreover, the sum of monomers (bound acids) was much higher than that of
dehydrodiferulic acids (DiFA).
FUNDING
We thank ENSA, El-Harrach, Algiers and INRAE of
Bordeaux, France for their financial support within the
framework of the “CMEP PHC TASSILI” Project.
RESPONSIBLE EDITOR
Piotr Kaczyński
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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