ORIGINAL ARTICLE
Chili (Capsicum annuum L.) genotypes resistant to Pepper yellow leaf curl Thailand virus (PepYLCTHV)
1
Plant Production of Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand
2
Department of Plant Medicine, College of Agriculture, National Chiayi University, Chiayi, Taiwan
3
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
4
Pepper Breeding Section, Plant Geneticist and Breeder (Independent), India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2021-03-26
Acceptance date: 2021-08-09
Online publication date: 2021-12-20
Corresponding author
Patcharaporn Suwor
Plant Production of Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand
Plant Production of Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand
Journal of Plant Protection Research 2021;61(4):377-383
KEYWORDS
TOPICS
ABSTRACT
Pepper yellow leaf curl Thailand virus (PepYLCTHV) causes leaf curl disease in chili pro-
duction regions of the tropics and subtropics. Information on PepYLCTHV disease severi-
ty and resistance in chili pepper is still limited in Thailand. This study reports PepYLCTHV
disease severity through graft inoculation and selection of single resistant plants for use in
a chili breeding program. Twenty-one chili genotypes consisting of the local cultivar (5)
collected from Thailand, breeding lines (9) developed at Khon Kaen University (KKU),
Thailand and improved lines (7) obtained from the World Vegetable Center, Taiwan were
used in this study. Forty-five-day-old seedlings of all the genotypes were graft inoculated
with PepYLCTHV in a randomized complete block design (RCBD) with three replications
and 10 plants per replication and kept in a plastic net house. Disease symptoms were scored
at 20, 27, 34, 41 48, and 55 days after graft/inoculation (DAI). Disease severity was visually
recorded using 0−5 scores. Results showed that the disease severity of 21 chili genotypes
significantly differed at 48 days after grafting. High resistance and stability were shown by
9853-123 genotypes. Two genotypes, PSP11-7 and PSP11-10-1, showed resistant reaction
with disease severity scores of 1.9 and 1.8, respectively. However, among 21 chili geno-
types or 630 grafted plants, 302 plants were successfully grafted inoculated plants. There-
fore, from the results of this work, highly resistant plants (69 single plants) can be selected,
selfed and advanced for breeding.
ACKNOWLEDGEMENTS
We wish to thank the Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand, for experimental facilities support and the World Vegetable Center (WorldVeg), Taiwan, for providing germplasm.
FUNDING
This research was supported by the Center of Excellence on Agricultural Biotechnology, Science and Technology, Postgraduate Education and Research Development Office, Office of Higher Education Commission, Ministry of Higher Education, Science, Research and Innovation (Grant # AG-BIO/PERDO-CHE) and Academic Melting Pot Fiscal Year, 2020 of King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand.
RESPONSIBLE EDITOR
Przemysław Wieczorek
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (17)
1.
Anaya-López J.L., Torres-Pacheco I., González-Chavira M., Garzon-Tiznado J.A., Pons-Hernandez J.L. 2003. Resistance to geminivirus mixed infections in mexican wild peppers. Journal American Society Horticultural Science 38 (2): 251–255. DOI: https://doi.org/10.21273/HORTS....
2.
Barchenger D.W., Jeeatid N., Lin S.W., Wang Y.W., Lin T.H., Chan Y.L., Kenyon L. 2019. A novel source of resistance to Pepper yellow leaf curl Thailand virus (PepYLCThV) (Begomovirus) in chile pepper. Journal American Society Horticultural Science 54 (12): 2146−2149. DOI: https://doi.org/10.21273/HORTS....
3.
Chiemsombat P., Srikamphung B., Yule S., Srinivasan R. 2018. Begomoviruses associated to pepper yellow Leaf curl disease in Thialand. Journal of Agricultural Research 3 (7): 000183.
4.
Food and Agriculture Organization. 2017. Agricultural Production: Primary crops. Available on: http://apps.fao.org. [Accessed: 25 January 2020].
5.
Kumar R., Rai N., Kakpale N. 1999. Field reaction of some chilli genotypes for leaf curl virus in Chhattisgarh region of India. The Orissa Journal of Horticulture 27: 100−102. DOI: https://doi.org/10.18782/2320-....
6.
Kumar S., Kumar S., Singh M., Singh A.K., Rai M. 2006. Identification of host plant resistant to pepper leaf curl virus in chilli (Capsicum species). Scientia Horticulturae 110: 359−361. DOI: https://doi.org/10.1016/j.scie....
7.
Kumar S., Kumar R., Kumar S., Singh A.K., Singh M., Rai A.B., Rai A.B. 2011. Incidence of leaf curl disease on capsicum germplasm under field conditions. Indian Journal of Agricultural Sciences 81: 187−189.
8.
Mishra M.D., Raychaudhuri S.P., Jha A. 1963. Virus causing leaf curl of chilli (Capsicum annuum L.). International Journal of Microbiology 3: 73–76.
9.
Rai V.P.R., Kumar S., Singh P., Kumar S., Singh M., Rai M. 2014. Monogenic recessive resistant to pepper by leaf curl virus in an interspecific cross of Capsicum. Scientia Horticulturae 172: 34−38. DOI: https://doi.org/10.1016/j.scie....
10.
Sakata J.J., Shibuya Y., Sharma P., Ikegami M. 2008. Strains of a new bipartite begomovirus, Pepper yellow leaf curl Indonesia virus, in leaf-curl-diseased tomato and yellow-vein diseased ageratum in Indonesia. Archives of Virology 153 (12): 2307−2313. DOI: https://doi.org/10.1007/s00705....
11.
Sangsotkaew Y., Jeeartid N., Siri N., Thummabenjapone P., Chatchawankanphanich O., Phuangrat B., Techawongstien S. 2018. Phenotypic responses of putative resistance chili cultivars infected by PepLCV with viruliferous whitefly transmission. Acta Horticulturae 67. DOI: https://doi.org/10.18690/978-9....
12.
Shih S.L., Tsai W. S., Lee L.M., Wang J.T., Green S.K., Kenyon L. 2010. First report of tomato yellow leaf curl Thailand virus associated with pepper leaf curl disease in Taiwan. Plant Disease 94 (5): 637. DOI: https://doi.org/10.1094/PDIS-9....
13.
Srivastava A., Mangal M., Saritha R.K., Kalia P. 2017. Screening of chilli pepper (Capsicum spp.) lines for resistance to the Begomovirus causing chili leaf curl disease in India. Journal of Crop Protection 100: 177–185. DOI: https://doi.org/10.1016/j.crop....
14.
Tsai W., Shih S., Green S., Rauf A., Hidayat S., Jan F.J. 2006. Molecular characterization of Pepper yellow leaf curl Indonesia virus in leaf curl and yellowing diseased tomato and pepper in Indonesia. Plant Disease 90 (2): 247−247. DOI: https://doi.org/10.1094/PD-90-....
15.
Tsai W.S., Shih S.L., Kenyon L., Green S.K., Jan F.J. 2011. Temporal distribution and pathogenicity of the predominant tomato-infecting begomoviruses in Taiwan. Plant Pathology 60: 787−799. DOI: https://doi.org/10.1111/j.1365....
16.
Verlaan M.G., Hutton S.F., Ibrahem R.M., Kormelink R., Visser R.G.F., Scott J.W., Edwards J.D., Bai Y. 2013. The tomato yellow leaf curl virus resistance genes Ty-1 and Ty-3 are allelic and code for DFDGD-Class RNA–Dependent RNA polymerases. PLoS Genetics 9 (3): e1003399. DOI: https://doi.org/10.1371/journa....
17.
Zehra S.B., Ahmad A., Sharma A., Sofi S., Lateef A., Bashir Z., Husain M., Rathore J.P. 2017. Chilli leaf curl virus an emerging threat to chilli in India. Indian Journal of Pure and Applied Biosciences 5 (5): 404−414.
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
