ORIGINAL ARTICLE
Chemical constituents and ovicidal effects of mahlab, Prunus mahaleb L. kernels oil on cotton leafworm, Spodoptera littoralis (Boisd.) eggs
1
Plant Protection Research Institute, Agricultural Research Center, Dokki, 44516 Giza, Egypt
Submission date: 2016-04-01
Acceptance date: 2016-08-19
Corresponding author
Hala M. Mead
Plant Protection Research Institute, Agricultural Research Center, Dokki, 44516 Giza, Egypt
Plant Protection Research Institute, Agricultural Research Center, Dokki, 44516 Giza, Egypt
Journal of Plant Protection Research 2016;56(3):279-290
KEYWORDS
TOPICS
ABSTRACT
The carried out investigations evaluated ovicidal activity of mahlab, Prunus mahaleb
L. kernel oil against cotton leafworm, Spodoptera littoralis (Boisd.). The chemical constituents of the fixed oil of mahlab were analyzed using gas-liquid chromatography (GLC). Timnodonic (33.07%), oleic (28.71%) and linoleic (24.35%) were the basic fatty acids, while the major hydrocarbon and sterol were found to be heneicosane (62.57%) and β-sitosterol (10.57%). The LC 50 values for the one-day-old egg masses were found to be more susceptible than 3-day-old ones. Moreover, the leaf dip technique occurred to be more efficient than spraying technique. The results also showed abnormalities in the external morphology of egg shell, chorion surface, shell imprints and aeropyles of
S. littoralis eggs treated with mahlab and KZ oils as compared to a control by using scanning electron microscope. Generally, the tested oils significantly reduced the activities of transaminase enzymes (AST and ALT), acid and alkaline phosphatases and total soluble protein except mahlab oil on acid phosphatase as compared to a control. Additionally, the oils of both mahlab and KZ oil affected some biological aspects such as incubation period, larval duration, larval mortality and pupal weight comparing to a control.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (39)
1.
Abbott W.S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18 (2): 265–267.
2.
Abdel-Hafez M.M., Shaaban M.N., El-Malla M.A, Farag M., Abdel-Kawy A.M. 1988. Effect of insect growth regulators on the activity transaminases enzymes with reference to protein and amino acids in the Egyptian cotton leafworm, Spodoptera littoralis (Boisd.). Minia Journal of Agriculture Research and Development 10 (3): 1357–1372.
3.
Adedire C.O., Ajayi O.E. 2003. Potential of sandbox, Hura crepitans L. seed oil for protection of cowpea seeds from Callosobruchus maculatus Fabricius (Coleoptera: Bruchidae) infestation. Journal of Plant Diseases and Protection 110 (6): 602–610.
4.
Arens M., Schulte E., Weber K. 1994. Fettsäuremethylester, umesterung mit trimethylsulfoniumhydroxid (schnellverfahren) [Fatty-acid methylesters, transesterification with trimethyl sulfonium hydroxide (rapid method)]. Fett Wissenschaft Technologie 96 (2): 67–68. (in Germany).
5.
Chapman R.F. 1998. The Insects Structure and Function. Cambridge University Press, New York, USA, 770 pp.
6.
Chino H., Yushima T. 1953. On the occurrence of an acetylcholine-like substance in some insect eggs (2). The change in acetylcholine-like substance content during embryonic development in some insect eggs. Annotationes Zoologicae Japonenses 26 (4): 233–237.
7.
Costat Statistical Software 2005. Microcomputer program analysis version, 6. 311. CoHort Software, Monterey, California, USA.
8.
Crabtree B., Newsholme E.A. 1970. The activities of proline dehydrogenase, glutamate dehydrogenase, aspartate-oxoglutarate aminotransferase and alanine-oxoglutarate aminotransferase in some insect flight muscles. Biochemical Journal 117 (5): 1019–1021.
9.
Cunniff P. 1995. Official Methods of Analysis of the Association of Official Analytical Chemists International. 16th ed., Association of Official Analytical Chemists, Arlington, Virginia, USA, 1141 pp.
10.
Don-Pedro K.N. 1989. Mode of action of fixed oils against eggs of Callosobruchus maculatus (F.). Pest Management Science 26 (2): 107–115.
11.
El-Defrawi M.E., Toppozada A., Mansour N., Zeid M. 1964. Toxicological studies on the Egyptian cotton leafworm, Prodenia litura. I. susceptibility of different larval instars of Prodenia to insecticides. Journal of Economic Entomology 57 (4): 591–593.
12.
El-Kordy M.W., Gadallah A.I., Abbas M.G., Mostafa S.A. 1995. Effect of pyriproxyfen, flufenoxuron and teflubenzuron on some biochemical aspects of Spodoptera littoralis. Al-Azhar Journal of Agricultural Research 21: 223–238.
13.
El-Sweerki F.I. 2002. Ovicidal activity of natural and synthetic compounds against cotton leafworm. Ph.D. thesis, Institute of Environmental Studies and Research, Ain Shams University, Egypt, 157 pp.
14.
Etebari K., Mirhoseini S.Z., Matindoost L. 2005. A study on interaspecifc biodiversity of eight groups of silkworm (Bombyx mori) by biochemical markers. Insect Science 12 (2): 87–94.
15.
Finney D.J. 1952. Probit Analysis: a Statistical Treatment of the Sigmoid Response Curve. 2nd ed. Cambridge University Press, NewYork – London, 318 pp.
16.
Gornall A.G., Bardawill C.J., David M.M. 1949. Determination of serum proteins by means of biuret reaction. Journal of Biological Chemistry 177 (2): 751–766.
17.
Helmy E.I., Kwaiz F.A., El-Sahn O.M.N. 2012. The usage of mineral oils to control insects. Egyptian Academic Journal of Biological Sciences 5 (3): 167–174.
18.
Helmy E.I., Hanafy H.A., Hassan N.A., El-Imery S.M., Mohamed F.A. 1992. New approach to control scale insects by using five Egyptian miscible oils on orange trees in Egypt. Egyptian Journal of Agricultural Research 70 (3): 763–770.
19.
Hosny M.M., Isshak R.R. 1967. New approaches to the ecology and control of three major cotton pests in U. A. R. Part 1: Factors stimulating the outbreaks of the cotton leafworm in U. A. R. and the principle of its predication. Technical Bulletin of Ministry of Agriculture United Arab Republic 1: 1–36.
20.
Kamel A.M. 2010. Can we use the moringa oil as botanical insecticide against Spodoptera frugiperda? Academic Journal of Entomology 3 (2): 59–64.
21.
Khedr M.A., Al-Shannaf H.M., Mead H.M., Shaker S.A. 2015. Comparative study to determine food consumption of cotton leafworm, Spodoptera littoralis, on some cotton genotypes. Journal of Plant Protection Research 55 (3): 312–321.
22.
Khedr M., El-Kawas H. 2013. Control of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) and Tetranychus urticae Koch (Acari: Tetranychiclae) by coriander essential oil. Journal of Entomology 10 (4): 170–181.
23.
Malek M.A., Wilkins R.M. 1994. Toxicity of Annona squamosa Linn. seed oil extract on Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). p. 819–823. In: Proceedings of the 6th International Working Conference on Stored-product Protection (E. Highley, E.J. Wright, H.J. Banks, B.R. Champ, eds.). Canberra, Australia, 17–23 April 1994. CAB International, Wallingford, UK, 1274 pp.
24.
Marei S.S., Amr E.M., Salem N.Y. 2009. Effect of some plant oils on biological, physiological and biochemical aspects of Spodoptera littoralis (Boisd.). Research Journal of Agriculture and Biological Sciences 5 (1): 103–107.
25.
Mariod A.A., Aseel K.M., Mustafa A.A., Abdel-Wahab S.I. 2009. Characterization of the seed oil and meal from Monechma ciliatum and Prunus mahaleb seeds. Journal of the American Oil Chemists’ Society 86 (8): 749–755.
26.
Mariod A.A., Ibrahim R.M., Ismail M., Ismail N. 2010. Antioxidant activities of phenolic rich fractions (PRFs) obtained from black mahlab (Monechma ciliatum) and white mahlab (Prunus mahaleb) seedcakes. Food Chemistry 118 (1): 120–127.
27.
Mehrotra K.N. 1960. Development of the cholinergic system in insect eggs. Journal of Insect Physiology 5 (2): 129–142.
28.
Meister A. 1957. Biochemistry of the Amino Acids. p. 175–196. Academic Press, New York, USA, 485 pp.
29.
Özçelik B., Koca U., Kaya D.A., Şekeroğlu N. 2012. Evaluation of the in vitro bioactivities of mahaleb cherry (Prunus mahaleb L.). Romanian Biotechnological Letters 17 (6): 7863–7872.
30.
Perumalsamy H., Jang M.J., Kim J.R., Kadarkarai M., Ahn Y.J. 2015. Larvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in Millettia pinnata seed toward three mosquito species. Parasites and Vectors 8 (1): 237.
31.
Powell M.E.A., Smith M.J.H. 1954. The determination of serum acid and alkaline phosphatase activity with 4-aminoantipyrine (A.A.P.). Journal of Clinical Pathology 7 (3): 245–248.
32.
Ramadan M.F., Mörsel J.T. 2003. Oil goldenberry (Physalis peruviana L.). Journal of Agricultural and Food Chemistry 51 (4): 969–974.
33.
Rauschenbach I.Y., Bogomolova E.V., Gruntenko N.E., Adonyeva N.V., Chentsova N.A. 2007. Effects of juvenile hormone and 20-hydroxyecdysone on alkaline phosphatase activity in Drosophila under normal and heat stress conditions. Journal of Insect Physiology 53 (6): 587–591.
34.
Regnault-Roger C. 1997. The potential of botanical essential oils for insect pest control. Integrated Pest Management Reviews 2 (1): 25–34.
35.
Reitman S., Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology 28 (1): 56–63.
36.
Shams K.A., Schmidt R. 2007. Lipid fraction constituents and evaluation of anti-anaphylactic activity of Prunus mahaleb L. Kernels. African Journal of Traditional, Complementary and Alternative Medicines 4 (3): 289–293.
37.
Snedecor G.W., Cochran W.G. 1980. Statistical Methods. 7th edition. Iowa State University Press, Ames, Iowa, USA, 507 pp.
38.
Sridhara S., Bhat J.V. 1963. Alkaline and acid phosphatases of the silkworm, Bombyx mori L. Journal of Insect Physiology 9 (5): 693–701.
39.
Tsumuki H., Kanehisa K. 1984. Phosphatases in the rice stem borer, Chilo supperssalis Walker (Lepidoptera: Pyralidae): Some properties and changes of the activities during hibernation. Cryobiology 21 (2): 177–182.
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