ORIGINAL ARTICLE
Characterization of phytoplasmas related to aster yellows group infecting annual plants in Iran, based on the studies of 16S rRNA and rp genes
1
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, 84156, Isfahan, Iran
Submission date: 2013-10-12
Acceptance date: 2014-01-01
Corresponding author
Leila Zirak
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, 84156, Isfahan, Iran
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, 84156, Isfahan, Iran
Journal of Plant Protection Research 2014;54(1):1-8
KEYWORDS
TOPICS
ABSTRACT
Several annual field crops, vegetables, ornamentals, oilseed crops, and weeds showing phytoplasma diseases symptoms were collected to detect phytoplasmas related to‘
Candidatus Phytoplasma asteris’. The collecting was done in the central regions of Iran. For general detection of phytoplasmas, 16S rRNA gene fragments were amplified using phytoplasma universal primer pair P1/P7 in polymerase chain reaction (PCR) followed by primer pair R16F2n/R16R2 in nested PCR. Then, for finer detection of phytoplasmas related to ‘Ca. P. asteris’, DNA samples were used to extend the rp and tuf gene fragments by PCR using aster yellows group specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment lenght polymorphism (RFLP) analysis of
rp gene fragments using digestion with Alu I, Mse I, and Tsp 509I restriction enzymes indicated that aster yellows group related phytoplasmas in these Iranian regions, belong to rp I-B subgroups. Sequence analysis of partial 16S rRNA and rp genes from representative phytoplasma isolates confirmed the RFLP results. This research is the first report of annual plants infected with phytoplasmas related to subgroup rp I-B in Iran.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (18)
1.
Asghari Tazehkand S., Hosseini Pour A., Heydarnejad J., Massumi H., Azadvar M. 2010. Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman province. Iran. J. Plant Pathol. 158 (11–12): 713–720.
2.
Babaie G., Khatabi B., Bayat H., Rastgou M., Hosseini A., Salekdeh G.H. 2007. Detection and characterization of phytoplasma infecting ornamental and weed plants in Iran. J. Plant Pathol. 155 (6): 368–372.
3.
Cieślińska M. 2011. Less common phytoplasma infecting stone fruit trees. J. Plant Prot. Res. 51 (4): 435–440.
4.
Deng S., Hiruki C. 1991. Genetic relatedness between two nonculturable mycoplasma like organisms revealed by nucleic acid hybridization and polymerase chain reaction. Phytopathology 81 (12): 1475–1479.
5.
Girsova N., Bottner K.D., Mozhaeva K.A., Kastalyeva T.B., Owens R.A., Lee I.M. 2008. Molecular detection and identification of group 16SrI and 16SrXII phytoplasmas associated with diseased potatoes in Russia. Plant Dis. 92 (4): 654–661.
6.
Hosseini P., Bahar M., Madani G., Zirak L. 2011. Molecular characterization of phytoplasmas associated with potato purple top disease in Iran. J. Phytopathol. 159 (4): 241–246.
7.
Kunkel L.O. 1932. Celery yellows of California not identical with the aster yellows of New York. Contrib. Boyce Thompson Inst. 4: 405–414.
8.
Lee I.M., Gundersen-Rindal D.E., Davis R.E., Bottner K.D., Marcone C., Seemüller E. 2004. ‘Candidatus Phytoplasma asteris’, a novel phytoplasma taxon associated with aster yellows and related diseases. Int. J. Syst. Evol. Microbiol. 54 (4): 1037–1048.
9.
Lee I.M., Hammond R.W., Davis R.E., Gundersen D.E. 1993. Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasma-like organisms. Phytopathology 83 (8): 834–842.
10.
Marcone C., Lee I.M., Davis R.E., Ragozzino A., Seemüller E. 2000. Classification of aster yellows group phytoplasma based combined analysis of rRNA and tuf gene sequences. Int. J. Syst. Evol. Microbiol. 50 (5): 1703–1713.
11.
Martini M., Lee I.M., Bottner K.D., Zhao Y., Botti S., Bertaccini A., Harrison N.A., Carraro L., Marcone C., Khan A.J., Osler R. 2007. Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. Int. J. Syst. Evol. Microbiol. 57 (9): 2037–2051.
12.
Murray M.G., Thompson W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8 (19): 4321–4325.
13.
Rashidi M., Ghosta Y., Bahar M. 2010. Molecular identification of a phytoplasma associated with Russian olive witches’- broom in Iran. Eur. J. Plant Pathol. 127 (2): 157–159.
14.
Salehi M., Heydarnejad J., Izadpanah K. 2005. Molecular characterization and grouping of 35 phytoplasmas from central and southern provinces in Iran. Iran J. Plant Pathol. 41: 62–65.
15.
Schneider B., Seemüller E., Smart C.D., Kirkpatrick B.C. 1995. Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas: Molecular and diagnostic procedures in mycoplasmology. p. 369–380. In: “Molecular and Diagnostic Procedures in Mycoplasmalogy”. Vol. 1. (S. Razin, J.G. Tully, eds.). Academic Press Inc., San Diego, 451 pp.
16.
Vali Sichani F., Bahar M., Zirak L. 2011. Characterization of stolbur (16SrXII) group phytoplasmas associated with Cannabis sativa witches’-broom disease in Iran. Plant Pathol. J. 10 (4): 161–167.
17.
Zhao Y., Wei W., Lee I.M., Shao J., Suo X., Davis R.E. 2009. Construction of an interactive online phytoplasma classification tool, i PhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int. J. Syst. Evol. Microbiol. 59 (10): 2582–2593.
18.
Zirak L., Bahar M., Ahoonmanesh A. 2010. Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ and peanut WB group associated with sweet cherry diseases in Iran. J. Phytopathol. 158 (1): 63–65.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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