ORIGINAL ARTICLE
Characterization of a nucleopolyhedrovirus isolated from the labolatory rearing of the beet armyworm Spodoptera exigua (Hbn.) in Poland
1
Institute of Plant Protection, Miczurina 20,60-318 Poznań
2
Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
Journal of Plant Protection Research 2005;45(4):279-286
KEYWORDS
TOPICS
ABSTRACT
A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera
exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-X R , was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh , lef-8 and pif-2 , showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata(Maco) MNPV, and much less to SeMNPV (Spod-X R ).These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-X R was th emost inf ectious for S. exigua , while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (24)
1.
Allaway G.P., Payne C.C. 1984. Host range and virulence of five baculoviruses from lepidopterous hosts. Ann. Appl. Biol., 105: 29–37.
2.
Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990. Basic local alignment search tool. J. Mol. Biol., 215: 403–410.
3.
Cory J.S., Myers J.H. 2003. The ecology and evolution of insect baculoviruses. Ann. Rev. Ecol. Evol. Syst., 34: 239–272.
4.
Gelernter W.D., Federici B.A. 1986. Isolation, identification and determination of virulence of a nuclear polyhedrosis virus from the beet armyworm, Spodoptera exigua (Lepidoptera; Noctuidae). Environ. Entomol., 15: 240–245.
5.
Graham R.I., Tyne W.I., Possee R.D., Sait S.M., Hails R.S. 2004. Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney. J. Invertebr. Pathol., 87: 29–38.
6.
Herniou E.A., Olszewski J.A., O’Reilly D.R., Cory J.S. 2004. Ancient coevolution of baculoviruses and their insect hosts. J. Virol., 78: 3244–3251.
7.
IJkel W.F., van Strien E.A., Heldens J.G., Broer R., Zuidema D., Goldbach R.W., Vlak J.M. 1999. Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J. Gen. Virol., 80: 3289–3304.
8.
Jakubowska A., Oers M.M., Cory J.S., Ziemnicka J., Vlak J.M.2005a. European Leucoma salicis MNPV is closely related to North-American Orgyia pseudotsugata MNPV. J. Invertebr. Pathol., 88: 100–107.
9.
Jakubowska A., Oers M.M., Ziemnicka J., Lipa J.J., Vlak J.M. 2005b. Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland. J. Invertebr. Pathol., 89: 64–68.
10.
Kolodny-Hirsch D.M., van Beek N.A.M. 1997. Selection of a morphological variant of Autographa californica nuclear polyhedrosis virus with increased virulence following serial passage in Plutella xylostella. J. Invertebr. Pathol., 74: 103–111.
11.
nuclear polyhedrosis virus with increased virulence following serial passage in Plutella xylostella. J. Invertebr. Pathol., 74: 103–111.
12.
Li L., Donly C., Li Q., Willis L.G., Keddie B.A., Erlandson M.A., Theilmann D.A. 2002. Identification and genomic analysis of a second species of nucleopolyhedrovirus isolated from Mamestra configurata . Virology 5: 226–44.
13.
Lipa J.J., Śliżyński K. 1973. Wskazówki metodyczne i terminologia do wyznaczania średniej dawki smiertelnej (LD 50 ) w patologii owadów i toksykologii. Prace Nauk. Inst. Ochr. Roślin 15 (1): 59–83.
14.
Moraes R.R., Maruniak J.E. 1997. Detection and identification of multiple baculoviruses using the polymerase chain reaction (PCR) and restriction endonuclease analysis. J. Virol. Methods 63: 209–217.
15.
Muoz D., Vlak J.M., Caballero P. 1997. In vivo recombination between two strains of the genus Nucleopolyhedrovirus in its natural host, Spodoptera exigua . Appl. Environ.Microbiol., 63: 3025–3031.
16.
Muoz D., Murillo R., Krell P.J., Vlak J.M., Caballero P. 1999. Four genotypic variants of a Spodoptera exigua Nucleopolyhedrovirus (Se SP2) are distinguishable by a hypervariable genomic region. Virus Res., 59: 61–74.
17.
Murillo R., Muńoz D., Lipa J.J, Caballero P. 2001. Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae J. Appl. Entomol., 125: 267–270.
18.
Page R.D.M. 1996. TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357–358.
19.
Poitout S., Bues R. 1970. Élevage de plusieurs espèces de Lépidoptères Noctuidae sur milieu artificiel et sur milieu artificiel simplifié. Ann. Zool. Ecol. Anim., 2: 79–91.
20.
Reed C., Otvos I.S., Reardon R., Ragenovich I., Williams H.L. 2003. Effects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1. J. Invert. Pathol., 84: 104–113.
21.
Smits P.H. 1987.Nuclear polyhedrosis virus as biological control agent of Spodoptera exigua. Wageningen University Ph.D. Thesis, 127 pp.
22.
Smits P.H., Vlak J.M. 1988. Selection of nuclear polyhedrosis virus as biological control agent of Spodoptera exigua ( Lep.: Noctuidae ). Entomophaga 33: 299–308.
23.
Swofford D.L. 1998. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, MA. Tompkins G.J., Dougherty E.M., Adams J.R., Diggs D. 1988. Changes in the virulence of nuclear polyhedrosis viruses when propagated in alternate noctuids (Lepidoptera: Noctuidae) cell lines and hosts. J. Econ. Entomol., 81: 1027–1032.
24.
Vlak J.M., van Frankenhuizen K., Peters D., Gröner A. 1981. Identification of a new nuclear polyhedrosis virus from Spodoptera exigua . J. Invertebr. Pathol., 38: 297–298.
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
