ORIGINAL ARTICLE
Characterization of Fusarium isolates from rice, sugarcane and maize using RFLP-IGS
1
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia
Corresponding author
Zakaria Latiffah
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia
Journal of Plant Protection Research 2010;50(4):409-415
KEYWORDS
TOPICS
ABSTRACT
Isolates of Fusarium from rice, sugarcane and maize were identified as F. verticillioides, F. sacchari, F. proliferatum F. subglutinans, F. fujikuroi and F. oxysporum. The species were then characterized by restriction analysis of intergenic spacer (RFLP-IGS) using
AluI, Eco 88I, Rsa I and XhoI. Twenty-five haplotypes were identified among the isolates of Fusarium which indicated high levels of variations. UPGMA cluster analysis was conducted to cluster the isolates and to estimate the intraspecific and interspecific variability. Isolates of F. fujikuroi from rice were clustered together with isolates of F. proliferatum from rice and maize with a similarity value of 88–100%. Isolates of F. verticilliodes from maize and sugarcane were clustered together with a similarity value ranging from 92–100%, and two isolates from rice formed another cluster. Isolates of
F. oxysporum from maize and rice were clustered together with a similarity value ranging from 87–95%. Isolates of F. subglutinans from rice and maize, and F. sacchari from rice and sugarcane were also clustered together with a similarity value of 77–100%. Based on RFLP-IGS analysis, variability was observed within and between species of Fusarium from rice, maize and sugarcane and the technique could be use to complement morphological characterization and to determine genetic relationships between the species.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (25)
1.
Appel D.J., Gordon T.R. 1995. Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA. Exp. Mycol. 19 (2): 120–128.
2.
Booth C. 1971. The Genus Fusarium. Commonwealth Agricultural Bureau, Bucks, United Kingdom.
3.
Burgess L.W., Liddell C.M., Summerell B.A. 1988. Laboratory Manual for Fusarium. Research. Department of Plant Pathology and Agricultural Entomology, University of Sydney.
4.
Edel V., Steinberg C., Avelange I., Langueere G., Alabouvette C. 1995. Comparison of three molecular methods for the identification of Fusarium oxysporum strains. Phytopathology 85 (5): 579–585.
5.
Gerlach W., Nirenberg H. 1982. The genus Fusarium: A pictorial atlas. Mitt. Biol. Bundesanst. Land- u. forstwirtsch. Berl.-Dahlem 209: 1–405.
6.
Gortz A., Oerke E.C., Steiner U., Wallwijk C., de Vriees I., Dehne H.W. 2008. Biodiversity of Fusarium species causing ear rot in Germany. p. 617–621. In: Proc. 3rd International Fusarium Head Blight Symposium Suppl. B. 51. Szeged, Hungary.
7.
Guadet J., Julien J., Lafay J.F., Brygod Y. 1989. Phylogeny of some Fusarium species, as determined by large – subunit rRNA sequence comparison. Mol. Biol. Evol. 6 (3): 227–242.
8.
Hillis D.M., Dixon M.T. 1991. Ribosomal DNA: Molecular evolution and phylogenetic inference. Q. Rev. Biol. 66 (4): 411–453.
9.
Kim H.J., Choi Y.K., Min B.R. 2001. Variation of the Intergenic Spacer (IGS) region of ribosomal DNA among Fusarium oxysporum formae speciales. J. Microbiol. 39 (4): 265–272.
10.
Kosiak E.B., Holst-Jensen A., Rundberget T., Jaen M.T.G., Torp M. 2005. Morphological, chemical and molecular differentiation of Fusarium equiseti isolates from Norwegian cereals. Int. J. Food Microbiol. 99 (2): 195–206.
11.
Konstantinova P., Yli-Mattila T. 2004. IGS-RFLP analysis and de avelopment of molecular markers for identification of Fusarium poae, Fusarium langethiae, Fusarium sporotrichioides and Fusarium kyushuense. Int. J. Food Microbiol. 95: 321–331.
12.
Leslie J.F., Summerell B.A. 2006. The Fusarium Laboratory Manual. 1st ed. Blackwell.
13.
Marasas W.F.O., Kriek N.P.J., Wiggins V.M., Steyn I.S., Towers D.L., Hastie T.J. 1979. Incidence, geographical distribution and toxigenicity of Fusarium species in South African corn. Phytopathology 69: 1181–1185.
14.
Martin F.N. 1990. Variation in ribosomal DNA repeat unit with in single oospore isolates of the genus Pythium. Genome 33 (4): 585–591.
15.
Munkvold G.P. 2003. Epidemiology of Fusarium diseases and their mycotoxin in maize ears. Eur. J. Plant Pathol. 109 (7): 705–713.
16.
Muramoto M., Tashiro T., Minarnihasni H. 1993. Distribution of Fusarium moniliforme var. Subglutinans in Kagoshima Prefecture and its pathogenicity. J. Japanese Forest. Soc. 75 (1): 1–9.
17.
Nelson A.J., Eliss K.S., Avevalo G.E., Darlington L.C., Bailey B.A. 1997. Genetic characterization by RAPD analysis of isolates of Fusarium oxysporum f. sp. Erythroxyli associated with an emerging epidermic in Peru. Phytopathology 87 (12): 1220–1225.
18.
Nelson P.E., Toussoun T.A., Cook R.J. 1983. Fusarium Species. An Illustrated Manual for Identification. The Pennsylvania State University Press. University Park and London.
19.
Nirenberg H.I., O’Donnell K. 1998. New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90 (3): 434–458.
20.
O’Donnell K., Ciglenik E., Nirenberg H.I. 1998. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90: 465–493.
21.
O’Donnell K., Gherbawy Y., Schweighkofler W., Adler A., Prillinger H. 1999. Phylogenetic analyses of DNA sequence and RAPD data compared in Fusarium oxysporum and related species from maize. J. Phytopathol. 147 (7–8): 445–452.
22.
Patiño B., Mirete S., Vázquez C., Jiménez M., Rodríguez M.T., González-Jaén M.T. 2006. Characterization of Fusarium verticillioides strains by PCR-RFLP analysis of the intergenic spacer region of the rDNA. J. Sci. Food Agric. 86 (3): 429–435.
23.
Rohlf F.J. 2000. NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.1. Exeter, New York.
24.
Steenkamp E.T., Wingfield B.D., Desjardins A.E., Marasas W.F.O, Wingfield M.J. 2000. Cryptic speciation of Fusarium subglutinans. Mycologia 94 (6): 1032–1043.
25.
Stephens C.T., de Vries R.H., Sink K.C. 1989. Evaluation of asparagus species for resistance to Fusarium oxysporum f. sp. Asparagi and Fusarium moniliforme. Am. Soc. Hort. Sci. 24 (2): 365–368.
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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