ORIGINAL ARTICLE
Changes in cellular immune responses of Chilo suppressalis Walker (Lepidoptera: Crambidae) due to pyriproxyfen treatment
1
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, 416351314 Iran
2
Biological Control Department, Iranian Institute of Plant Protection, Amol, 91951-46191 Iran
Submission date: 2015-03-23
Acceptance date: 2015-06-17
Corresponding author
Arash Zibaee
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, 416351314 Iran
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, 416351314 Iran
Journal of Plant Protection Research 2015;55(3):287-293
KEYWORDS
TOPICS
ABSTRACT
The effects of pyriproxyfen were determined on the cellular immunity and phenoloxidase activity in the 4th instar larvae of
Chilo suppressalis
Walker. The bioassay results revealed the effective concentrations of: 10L : 18C, 30L : 72C and 50L : 190C μg · ml
–1
. The
sole effect of 18 and 72 μg · ml
–1
concentrations at intervals of 1–3 h caused a higher number of total hemocytes in the treated larvae
than the control, but the reverse results were observed after 6–24 h. The number of plasmatocytes was lower than that of the control
for intervals of 3–24 h but the number of granulocytes was higher than the control after 1–3 h although no significant differences were
observed at the other times. In the treated larvae, the activities of phenoloxidase were higher and lower than those of the control after
1–3 h and 6–24 h, respectively. The combined effects of pyriproxyfen and the entomopathogenic fungus,
Beauveria bassiana
isolate B3
caused higher numbers of total hemocytes, plasmatocytes, and granulocytes in the treated larvae by use of the three concentrations of
pyriproxyfen, at intervals of 6 and 12 h. Although the numbers of nodules in the larvae treated with concentrations of 18 μg · ml
–1
were
higher than those of other treatments, the overall numbers were lower than those of the control. Finally, the activity of phenoloxidase
in the treated larvae was higher than that of the control, at intervals of 6 and 12 h post-treatment. Findings of the current study indicate
an intervening role of pyriproxyfen in the cellular immunity of
C. suppressalis
to entomopathogenic objects.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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