ORIGINAL ARTICLE
Canola seeds coating with formulations based on sodium alginate, chitosan and Thichoderma harzianum
1
Faculty of Agricultural Sciences, Institute of Research on Agricultural Production, Environment, and Health (IIPAAS), National University of Lomas de Zamora, Llavallol, Argentina
2
Phytotechnical Institute of Santa Catalina, National University of La Plata, La Plata, Argentina
3
Faculty of Agricultural Sciences, National University of Lomas de Zamora, Llavallol, Argentina
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-11-02
Acceptance date: 2024-01-08
Online publication date: 2024-03-06
Corresponding author
Cyntia Lorena Szemruch
Faculty of Agricultural Sciences, Institute of Research on Agricultural Production, Environment, and Health (IIPAAS), National University of Lomas de Zamora, Llavallol, Argentina
Faculty of Agricultural Sciences, Institute of Research on Agricultural Production, Environment, and Health (IIPAAS), National University of Lomas de Zamora, Llavallol, Argentina
Journal of Plant Protection Research 2024;64(1):61-68
HIGHLIGHTS
- Seed production demands the replacement of pesticides with natural products.
- This work provides a guide for seed producers in adopting biopolymer-based coatings.
- Canola seed can be coated with sodium alginate, chitosan, and Trichoderma harzianum.
- Some formulations maintain canola seed quality and safe storage.
KEYWORDS
TOPICS
ABSTRACT
Seed coating technology combined with biopolymers offers an alternative method to reduce
environmental contamination. However, when biological agents are incorporated,
biopolymers would have diverse properties and effects. This underscores the necessity of
exploring the optimal dosages and formulations of biopolymers to ensure the survival of
beneficial microorganisms, seed quality, and proper storage. This study aimed to explore
the effects of different sodium alginate and chitosan coating formulations on Trichoderma
harzianum viability and canola seeds quality. The coating process involved mixing T. harzianum
powder with sodium alginate, talc and chitosan in different doses, sequences and
formulations. Trichoderma harzianum viability was assessed through colony-forming units
per ml over time. Canola seed quality was evaluated by measuring radicle emergence, germination
percentage, seedling growth, and field emergence. Sodium alginate, both alone
and in combination with talc, improved T. harzianum viability immediately after treatment
and during storage. These coatings did not impair seed germination and improved canola
root growth. Among the different chitosan formulations, a 1 : 100 ratio in talc improved
strain survival and root growth without affecting germination, radicle, and field emergence.
Coating canola seeds is a practical alternative to the application of T. harzianum, sodium alginate
and talc, as it preserves their viability over time and improves seedling performance.
Chitosan formulations in acetic acid should be carefully developed to prevent negative effects
on seeds or biological agents.
ACKNOWLEDGEMENTS
We express our gratitude to LomasCyt Program – National
University of Lomas de Zamora, Argentina for
financial support and the Nuseed Company for the
contribution of the genetic material.
RESPONSIBLE EDITOR
Jolanta Kowalska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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