ORIGINAL ARTICLE
Burkholderia sp. strain TNAU-1 for biological control of root rot in mung bean (Vigna radiata L.) caused by Macrophomina phaseolina
1
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University
Coimbatore – 641 003, Tamil Nadu, India
Corresponding author
Rethinasamy Velazhahan
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University Coimbatore – 641 003, Tamil Nadu, India
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University Coimbatore – 641 003, Tamil Nadu, India
Journal of Plant Protection Research 2011;51(3):273-278
KEYWORDS
TOPICS
ABSTRACT
The potential of
Burkholderia
sp. strain TNAU-1
for the management of mung bean (
Vigna radiata
L.) root rot caused by
Macrophomina phaseolina
was evaluated under greenhouse conditions.
Burkholderia
sp. strain TNAU-1 inhibited the mycelial growth
of
M. phaseolina
in vitro
and produced an inhibition zone of 18.8 mm. Mung bean seeds when treated with the bacterial suspension,
showed significant increase in root length, shoot length and seedling vigour. A talc-based powder formulation of
Burkholderia
sp.
strain TNAU-1
was developed and evaluated for its efficacy in the management of mung bean root rot under greenhouse conditions.
Seed treatment or soil application of the powder formulation of
Burkholderia
sp. strain TNAU-1
significantly reduced the incidence of
root rot and increased the germination percentage and plant height. Seed treatment with the powder formulation of
Burkholderia
sp.
strain TNAU-1
alone was effective in controlling root rot disease; but the combined seed treatment and soil application of
Burkholderia
sp. strain TNAU-1, increased the efficacy. Seed treatment and soil application with
Burkholderia
sp. reduced the root rot incidence from
52.6 per cent (with non-bacterized seeds) to 16.7 per cent. Control of root rot with the application of
Burkholderia
sp. by seed treatment
and soil application was not statistically different from that obtained with seed treatment with carbendazim. The endophytic move-
ment of
Burkholderia
sp. in the stem, roots and leaves of mung bean was confirmed through PCR using
Burkholderia
sp. specific primers
which resulted in the amplification of a 417 bp product.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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