ORIGINAL ARTICLE
Biocontrol of Sphaerotheca fuliginea, the causal agent of powdery mildew of cucumber by using aqueous extracts from five traditional Egyptian medicinal plants
1
Department of Botany and Microbiology, Faculty of Science, University of Damietta, New Damietta, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-08-23
Acceptance date: 2022-10-14
Online publication date: 2023-02-01
Corresponding author
Zakaria Awad Baka
Department of Botany and Microbiology, Faculty of Science, University of Damietta, New Damietta, Egypt
Department of Botany and Microbiology, Faculty of Science, University of Damietta, New Damietta, Egypt
Journal of Plant Protection Research 2023;63(1):39-49
HIGHLIGHTS
- The powdery mildew disease of Cucurbitaceae caused a big problem in Egypt in reducing the yield of cucumber fruits.
- Aqueous extracts from Curcuma longa are efficient to control the powdery mildew disease caused by Sphaerotheca fuliginea.
- These bioactive compounds which are present in these plants may induce resistance against the pathogen>
KEYWORDS
TOPICS
ABSTRACT
The goal of this study was to evaluate the effectiveness of aqueous extracts from five traditional
Egyptian medicinal plants in preventing Sphaerotheca fuliginea’s powdery mildew
disease, which affects cucumber plants. Aqueous extracts from each of the examined plants
suppressed the pathogen’s conidia germination in vitro. In trials using detached leaves and
greenhouses, these extracts lessened the severity of the disease. Compared to other plant
extracts, Curcuma longa rhizome extract showed the greatest potency against the pathogen.
The aqueous extract of Curcuma longa showed the largest improvement in disease
suppression compared to the control in the greenhouse experiment. The results showed
that total phenol and associated defense enzyme levels (POD and PPO) were elevated
by plant extracts from all studied plants. These findings might suggest that total phenol
and associated defense enzymes strengthen the cucumber’s resistance to the disease. The
C. longa extract had more total phenol than the extracts from the other plants. The phenolic
components in the C. longa rhizome extract were varied, and these variations were detected
and quantified using high-performance liquid chromatography (HPLC). The content of
curcumin (3220.8 μg · g–1 dry weight) was the highest. In comparison to the control, the
foliar application of the C. longa extract considerably increased the cucumber fruit yield
and its constituent parts. This is the first time, to my knowledge, that the C. longa rhizome
extract has been utilized to improve cucumber plants’ production and its constituent parts.
The pathogen appeared as small colonies with fewer mycelia and immature conidia in the
treated cucumber leaves with 20% of C. longa rhizome extract according to an examination
by SEM. Overall, the results indicated that the extract of C. longa rhizome, was a promising,
effective, and environmentally friendly management measure against powdery mildew
disease of cucumbers, and thus could be used in the production of organically grown
vegetables.
ACKNOWLEDGEMENTS
The author would like to thank the University of
Damietta, Egypt for financial support to complete this
work.
RESPONSIBLE EDITOR
Jolanta Kowalska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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