ORIGINAL ARTICLE
Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat
1
Department of Production, Protection, and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, Morocco
2
Department of Veterinary, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, Morocco
3
FungiSem, IRHS, INRA-University of Angers-Agrocampus-Ouest, Angers, France
4
Laboratory of Phytopathology and Post-Harvest Quality, Regional Centre for Agronomic Research, Kénitra, Morocco
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-09-02
Acceptance date: 2022-10-27
Online publication date: 2022-09-03
Corresponding author
Ilham Barakat
Department of Production, Protection, and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, Morocco
Department of Production, Protection, and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, Morocco
Journal of Plant Protection Research 2023;63(1):59-67
HIGHLIGHTS
- In this study, we developed a novel method to evaluate the antibiosis effect of Trichoderma harzianum A through the use of the nephelometry technique. The results of our study confirmed the efficacy of the methodology adopted and the importance of this novel T. harzianum strain.
KEYWORDS
TOPICS
ABSTRACT
In this study Trichoderma harzianum strain A was isolated from the rhizosphere of an
argan tree in southern Morocco. Trichoderma harzianum strain A had previously demonstrated
a high antagonistic potential in vitro by direct confrontation and in vivo on wheat
plants in pots under greenhouse conditions against Zymoseptoria tritici, the agent of septoria
leaf blotch. In this study, the activity of filtrates prepared from the liquid culture of
T. harzianum A alone and from the confrontation medium with two Z. tritici strains [G1-1
(durum wheat) and A5-1 (soft wheat)] on the inhibition of Z. tritici pycnidiospore germination
was studied by nephelometry. The results of the antibiosis assay revealed that filtrate
0 (A in confrontation with G1-1) and F3 (A against A5-1) showed 95% of G1-1 and A5-1
pycnidiospore inhibition at 9/10 dilution of the undiluted filtrates after 4 days of incubation.
To understand and explain the antifungal activity of these filtrates, the extraction and
identification of secondary molecules of peptaibiotic nature secreted by T. harzianum A in
the three studied filtrates were performed. According to the results of high-performance
liquid chromatography-mass spectrometry (HPLC-MS) analyses, 38 peptaibiotic molecules
reported in the literature for their antifungal activity were identified in the different
extracts at high concentrations (high peak intensities). These molecules are divided
into nine groups, namely: Trichocryptin, Trichobrevin, Triochocryptin, Hypocompactin,
Hyporodicin, Trichocompactin, Alamethicine, Trichoferin, and Trichokonin. It was also
shown that the presence of the pathogen induces the production of peptaibols by the antagonistic
strain of Trichoderma.
ACKNOWLEDGEMENTS
The authors would like to thank the Ministry of National
Education, Vocational Training, Higher Education,
and Scientific Research of the Kingdom of
Morocco for funding the Arimnet research project
entitled “Bacplant,” and, we acknowledge the French
ANR (Agence National de la Recherche) project Lab
-Com, ESTIM for the financial support.
RESPONSIBLE EDITOR
Andrea Toledo
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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