ORIGINAL ARTICLE
Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight
1
Department of Biological Sciences, Higher Teacher Training College, University of Yaounde 1, Yaounde, Cameroon
2
Department of Biochemistry, Faculty of Sciences, University of Douala, Douala, Cameroon
3
Department of Microbiology, Faculty of Science, University of Yaoundé 1, Yaounde, Cameroon
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-03-29
Acceptance date: 2023-07-06
Online publication date: 2023-08-11
Corresponding author
Samuel Arsene Ntyam Mendo
Department of Biological Sciences, Higher Teacher Training College, University of Yaounde 1,Yaounde, Cameroon
Department of Biological Sciences, Higher Teacher Training College, University of Yaounde 1,Yaounde, Cameroon
Journal of Plant Protection Research 2023;63(3):350-365
HIGHLIGHTS
- This paper talks about friendly environmental fight against Phytophthora colocasiae, using fluorescent Pseudomonas rhizobacteria. It is research article providing new outcomes about immunization and growth stimulation of taro by Pseudomonas rhizobacteria in Cameroon. This is the first manuscript about the potential use of fluorescent Pseudomonas, in the fight against P. colocasiae.
KEYWORDS
TOPICS
ABSTRACT
Taro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat
to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant
growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth
promotion and suppress phytopathogens. In the present study, a total of 67 fluorescent
Pseudomonas spp. was characterized by 17.91, 5.97, and 4.47% populations of P. fluorescens,
P. chlororaphis, and P. putida, respectively, among the most represented. More than
36% of bacteria showed antagonistic potential through the production of both diffusible
and volatile compounds. Some of them (03) exhibited antagonistic activity in dual culture
against P. colocasiae with a diameter greater than 13 mm. These rhizobacteria produced
a significant amount of siderophore, IAA, SA, HCN, protease, lipases, and cellulases. For
the pot experiment, treatment by Pseudomonas significantly increased the enzymatic
activity involved in the resistance of taro, such as peroxidase (PO), polyphenol oxidase
(PPO), and phenylalanine ammonia-lyase (PAL). The two antagonists also increased plant
growth parameters of taro such as chlorophyll, plant height, shoot length, total leaf surface,
fresh root biomass, and fresh leaf biomass. These findings showed that fluorescent Pseudomonas
have an intriguing and undeniable potential in the fight against P. colocasiae, which
could lead to the development of a biopesticide in the future.
ACKNOWLEDGEMENTS
The authors are grateful to the University of Dschang
and the research unit of Natural Substances, (University
of Douala) for facilities and some consumables used
in this work.
RESPONSIBLE EDITOR
Krzysztof Krawczyk
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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