Allelopathy is a complex phenomenon which depends on allelochemical concentrations. So, two pot experiments were carried out to investigate the allelopathic effect of alcoholic fresh shoot extract of Eruca sativa (foliar spray) and E. sativa shoot powder (mixed with soil) on Pisum sativum plants and two associated weeds, Phalaris minor and Beta vulgaris. The experiments were conducted in the greenhouse of the National Research Centre, Giza, Egypt during two successive winter seasons (2016–2017 and 2017–2018). Ten treatments were applied in this study. Four treatments were applied before sowing, that E. sativa shoot powder was mixed with the soil at rates of 15, 30, 45 and 60 g ⋅ pot–1. The other four treatments of E. sativa alcoholic fresh shoot extract were sprayed twice on both plants and weeds at 5, 10, 15 and 20% (w/v) concentrations. Additionally, two untreated treatments, healthy (P. sativum only) and unweeded (untreated infested P. sativum plants with weeds) were applied for comparison. The results indicated that both alcoholic extracts and powder reduced growth of both weeds. Moreover, there was a direct relationship between concentration and weed reduction. Eruca sativa alcoholic extracts increased yield parameters of P. sativum plants. The maximum yield attributes were recorded by spraying of E. sativa alcoholic extract at 20%. On the other hand, it was clearly noticed that the high powder rates affected negatively P. sativum yield parameters. But the lowest powder rate (15 g ⋅ pot–1) stimulated P. sativum yield parameters as compared to unweeded treatment. Chemical analysis of E. sativa shoot powder ensured that the abundant amount of glucosinolates (9.6 μmol ⋅ g–1) and phenolic compounds (46.5 mg ⋅ g–1) may be responsible for its allelopathic effect. In conclusion, spraying of alcoholic fresh shoot extract of E. sativa at 20% (w/v) and mixing E. sativa shoot powder at 15 g · pot–1can be applied as natural bioherbicides for controlling weeds.