ORIGINAL ARTICLE
Acaricidal, ovicidal, and repellent effects of Tagetes patula leaf extract against Tetranychus urticae Koch (Acari: Tetranychidae)
1
Department of Vegetable and Ornamental Plant Mites, Plant Protection Research Institute, Agricultural Research Center (ARC), Cairo, Egypt
2
Department of Zoology, Suez Canal University, Ismailia, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-10-06
Acceptance date: 2019-05-17
Online publication date: 2019-06-13
Corresponding author
Manal Sayed Mohamed Ismail
Department of Vegetable and Ornamental Plant Mites, Plant Protection Research Institute, Agricultural Research Center (ARC), Cairo, Egypt
Department of Vegetable and Ornamental Plant Mites, Plant Protection Research Institute, Agricultural Research Center (ARC), Cairo, Egypt
Journal of Plant Protection Research 2019;59(2):151-159
KEYWORDS
TOPICS
ABSTRACT
This study investigates the acaricidal, ovicidal, and repellent effects of the Tagetes patula Linn.
(Asteraceae) leaf extract against both the adult female and egg stages of Tetranychus urticae Koch
(Trombidiformes: Tetranychidae) under laboratory conditions. The Tagetes patula ethanolic leaf
extract [TpEtOH70%] was screened for adulticide and ovicide bioassays in order to consider its acute
toxicity. One sublethal concentration was used to assess egg-laying capacity (fecundity), repellent,
and oviposition deterrent activities. The chemical characterization was conducted by gas
chromatography-mass spectrometry (GC-MS) analysis to identify the TpEtOH70% bioactive components.
Results showed that the LC50 value of TpEtOH70% leaf extract predicted by Probit analysis
against T. urticae adult females at 24 h was 0.99%. The TpEtOH70% leaf extract showed a significant
toxic effect as the highest mean mortality rates (± SE) of the treated adult females was 88.9 ± 3.7%.
However, the TpEtOH70% leaf extract was insignificant in affecting the egg-laying capacity of the
adult females treated with a sublethal dose of 0.5% even after 72 h. The TpEtOH70% leaf extract was
classified repellent since the repellent index (RI) value was lower than 1 – SD. In addition, it had
a high oviposition deterring effect based on a 100% reduction of the total number of eggs.
The TpEtOH70% leaf extract had a significant ovicidal effect on T. urticae eggs, with 56.04% reduction
in hatching. Five bioactive compounds from various classes of phytochemicals
were identified in the TpEtOH70% leaf extract and the major compound was phytol (62.72%).
This pioneering investigation reveals the adulticidal, ovicidal, and repellent activities of the
TpEtOH70% leaf extract against T. urticae. A combination of multiple modes of action of different
plant components may act alone or in synergism to delay the development of mite resistance.
ACKNOWLEDGEMENTS
We are thankful to Prof. Dr. Mona M.A. Ghallab, Vegetable
& Ornamental Plant Mites Department, Plant
Protection Research Institute (PPRI), Agricultural
Research Center (ARC), for her kind help and contribution
to this work. We would also like to extend our
thanks to the Orman Garden staff.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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